Table 3 of
Roger, Mol Vis 2007;
13:206-219.
Table 3. Genes isolated by subtractive suppressive hybridization in a DC6 (downregulated by ciliary neurotrophic factor after six days in vitro) library
DC6 subtraction suppressive hybridization library was generated using RNA from control explants as driver and the RNA from six days in vitro (6DIV)-ciliary neurotrophic factor treated explants as tester. Clone represents the cDNA number in the library; bp denotes the size in base pair of the cDNA; blast result indicates the published sequence identical to the clone sequence Symbol, Accession number, and Homologene refer to the available on-line datas for the published sequence. Status represents the cDNA characterization in this study: A for analyzed, R for rejected, no for gene that didn't exhibit gene expression variation, and V for gene validating the library. cDNA were classified accordingly to their gene ontology (GO).
Accession Clone bp Blast result Symbol number Homo Status ----- ---- -------------------------------- ------- ------------ ------ ------ GO: apoptosis D2 284 Caspase-3 Casp3 NM_012922 37912 V A7 258 Bcl-2 associated transcription Bclaf XM_214967 8832 A factor GO : Regulation of gene expression E2 693 Cone-Rod homeobox protein Crx NM_021855 467 V F2 521 Bromodomain adjacent zinc Baz2b XM_229225 8394 A finger domain 2b G4 574 t-complex associated testis Tcte1l NM_001013228 21304 no expressed 1 like C5 369 Riken cDNA 1500031M22 Dimt1 XM_00106535 0 7047 R D8 710 C21 ORF66 Isoform D N/A unknown 9604 R GO: Protein modification D1 465 Ste-20 related kinase Spak AF099990 22739 A C2 445 Ubiquitin Specific Peptidase 32 Usp32 NM_220798 13066 A B6 688 Acidic nuclear phosphoprotein 32 Anp32e NM_001013200 41519 A family member E B2 290 Protein tyrosine phosphatase Ptpip51 BC082081 34926 A interracting protein G7 1036 Epm2a interracting protein Epm2aip XM_236659 8875 A D4 240 Riken cDNA 5330408N05 gene N/A XM_23000 36420 R GO: Protein localization C7 490 Translocation protein 1 Tloc1 BC099207 2449 A GO : signal transduction A2 501 ADP-ribosylation factor 4 Arf4 NM_024151 55593 A F11 350 Homolog to TBC1 Domain family Tbc1d15 XM_345825 11249 R mb 15 GO: Cell Ion Homeostasis F5 670 Iron Responsive Element Ireb2 NM_022863 11280 no Binding protein 2 |