Table 2 of
Roger, Mol Vis 2007;
13:206-219.
Table 2. Genes isolated by subtractive suppressive hybridization in a UC6 (upregulated by ciliary neurotrophic factor after six days in vitro) library
UC6 subtraction suppressive hybridization library was generated using RNA from six days in vitro (6DIV)-ciliary neurotrophic factor treated explants as tester and the RNA from control as driver. cDNAs from this library were screened for differential expression and sequenced. Clone denotes the cDNA number in the library; bp represents the size in base pair of the cDNA; blast result indicates the published sequence identical to the clone sequence; symbol, accession number and Homo refer to the available homologene on-line datas for the published sequence. Status represents the cDNA characterization in this study: A for analyzed, R for rejected, no for gene that didn't exhibit gene expression variation, and V for gene validating the library.
Accession Clone bp Blast result Symbol number Homo Status ----- --- -------------------------------- ------ --------- ----- ------ 9H 234 Glial fibrillary acidic protein Gfap NM_017009 1554 V 6H 692 Pleiotrophin Ptn NM_017066 2117 A 4F 270 Oncostatin M receptor Osmr AB167522 2972 A 2G 370 Nuclear Receptor coactivator 1 Ncoa1 XM_233944 7859 no 10A 609 Glycyl t-RNA synthase Gars BC088347 1547 no 1H 486 RNA binding motif protein Rbmx unknown 20494 R 3B 367 Similar to RIKEN cDNA 3930401K13 N/A BC085931 12252 R |