Table 2 of
Yang, Mol Vis 2007;
13:2048-2057.Table 2 of
Yang, Mol Vis 2007;
13:2048-2057.
Table 2. Oligonucleotides used for reverse transcription-polymerase chain reaction in retinal microglial cells
Primers were designed using the Primer Express Program and purchased from AuGCT Biotechnology, Beijing, P. R. China.
Annealing Product
temperature size
Target gene Sequences (5'-3') Location (°C) (bp)
------------ -------------------------- ----------- ----------- -------
Eotaxin F: GCACGCTGAAAGCCATAGTCT NT 204-557 61 353
R: CATCTCCTTCCATGCTCTCTCCT
Rantes F: CTGCATCCCTCACCGTCATC NT 17-242 57 225
R: CACTTCTTCTCTGGGTTGGCA
IL-10 F: AGAAGCTGAAGACCCTCTGGA NT 385-693 59 308
R: TGGAGAGAGGTACAAACGAGGT
iNOS F: GCTCCATGACTCTCAGCACAG NT3164-3509 57 345
R: CTCAACCTGCTCCTCACTCAAG
β-Actin F: CATCCTGCGTCTGGACCT NT 603-1082 59 479
R: TCAGGAGGAGCAATGATCTTG
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