Table 1 of
Yang, Mol Vis 2007;
13:2048-2057.Table 1 of
Yang, Mol Vis 2007;
13:2048-2057.
Table 1. Oligonucleotides used for reverse transcription-polymerase chain reaction in murine photoreceptor (661W) cells in 661W cells
Primers were designed using the Primer Express Program and purchased from AuGCT Biotechnology, Beijing, P. R. China.
Annealing Product
temperature size
Target gene Sequences (5'-3') Location (°C) (bp)
------------ ------------------------- ----------- ----------- -------
MIP-1α F: CCAAAGAGACCTGGGTCCAAG NT 307-627 63 320
MIP-1β R: GGGTTGAGGAACGTGTCCTGA NT 76-463 62 387
F: CCATGAAGCTCTGCGTGTCTG
Eotaxin R: GGGCAGGAAATCTGAACGTG NT 139-419 61 280
F: CCTGCTGCTTTATCATGACCA
Rantes R: CCCTCAGAGCACGTCTTAGGA NT 57-366 57 309
F: TGCCCTCACCATCATCCTCA
IL-10 R: AAGCGATGACAGGGAAGCGTA NT 406-736 61 330
F: TCCCTGGGTGAGAAGCTGAAG
iNOS R: CGGAGAGAGGTACAAACGAGGT NT 402-818 57 416
F: CGACCCGTCCACAGTATGT
TNF-β R: TACAGTTCCGAGCGTCAAAG NT 546-875 63 329
F: AGCCGATGGGTTGTACCTTGT
β-Actin R: ACCCATTCCCTTCACAGAGCA NT 786-1031 62 245
F: CTGGAGAAGAGCTATGAGCTG
R: AATCTCCTTCTGCATCCTGTC
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