Table 2 of
Cui, Mol Vis 2007;
13:2023-2029.Table 2 of
Cui, Mol Vis 2007;
13:2023-2029.
Table 2. Gene specific primers for BFSP2
Eight gene specific primers (Invitrogen, Shanghai, China) were designed by Primer 3 software on line to screen the mutation in all 7 exons of BFSP2. F means forward strand, R means reverse strand.
Primer Sequence (5'-3') Length (bp) ------- -------------------- ----------- EXON1/F AATGCACAAACCCAAATGGT 587 EXON1/R AGGCCCTGAAGGAAGACACT EXON2/F GAGAGGCGAGTGGTAGTGGA 571 EXON2/R GGCCTCAGCCTACTCACAAC EXON3/F TGCAGACAGAGCATTTCCAC 690 EXON3/R GAGGGGTGTGAGCTGGATAA EXON3/F GCTGCAATTGCCTTCATTTT 526 EXON3/R GGGTAACCTGACCCACTTCA EXON4/F TCTGTGAAGCCTGTGTCTGG 666 EXON4/R CCCGGCCTCAATTATTCTTT EXON5/F ACCCAGGAGGAGGAAGTTGT 461 EXON5/R GGGAATCCCCTGGAAACTAA EXON6/F GGGGAATAGTCCAGGCTACC 458 EXON6/R ATGGGTGCCTATGTGAGAGG EXON7/F TTGTTCCAAAGGCCAGATTC 467 EXON7/R CACTCAAGGGAATCCTTCCA |