Table 1 of
Hansen, Mol Vis 2007;
13:2019-2022.
Table 1. PCR primer sequences and PCR conditions
Listed are each of the primer pairs used for PCR amplification of MAF. Included in the table is the length in base pairs (bp) of the generated fragment and the conditions used for each PCR reaction.
Fragment Primer name Primer sequence 5'-3' length PCR conditions ----------- ----------------------- -------- ---------------------------------- MAF_ex1.5f2 AGCTGGTGACCATGTCTGTG 407 bp Qiagen HotStart Taq + Q-solution: MAF_ex 1.5R AGAACTAGCAAGCCCACACC Predenat: 98°C, 5 min; denat: 96°C, 30 s; ann. 53.5°C, 30 s, ext. 72°C, 40 cycles MAF_ex 1.3F AACTGGCAATGAGCAACTCC 548 bp Qiagen HotStart Taq + Q-solution: MAF_ex 1.3R GTGGTGGTGGTGGTGGTAGT Predenat: 98°C, 5 min; denat. 96°C, 30 s; ann. 60°C, 30 s, ext. 72°C, 40 cycles MAF_ex1.2f2 GAGCGAGGGAGCACATTG 352 bp Qiagen HotStart Taq + Q-solution: MAF_ex 1.2r CCGGTTCCTTTTTCACTTCA Predenat: 98°C, 5 min; denat. 96°C, 30 s; ann. 60°C, 30 s, ext. 72°C, 40 cycles MAF_ex1.4f3 CCGCACTACCACCACCAC 432 bp Qiagen HotStart Taq + Q-solution: MAF_ex 1.4R CTGGTTCTTCTCCGACTCCA Predenat: 98°C, 5 min; denat. 96°C, 30 s; ann. 60°C, 30 s, ext. 72°C, 40 cycles MAF_ex2f AAATCCTGAGTAAGTGCCATTCA 575 bp New England Biolab Taq: MAF_ex2r GTTGCATTCCGGGAAACTT Predenat: 95°C, 5 min; denat. 95°C, 30 s; ann. 60°C, 30 s, ext. 72°C, 40 cycles |