Table 1 of Hansen, Mol Vis 2007; 13:2019-2022.


Table 1. PCR primer sequences and PCR conditions

Listed are each of the primer pairs used for PCR amplification of MAF. Included in the table is the length in base pairs (bp) of the generated fragment and the conditions used for each PCR reaction.

                                        Fragment
Primer name    Primer sequence 5'-3'     length              PCR conditions
-----------   -----------------------   --------   ----------------------------------
MAF_ex1.5f2   AGCTGGTGACCATGTCTGTG       407 bp    Qiagen HotStart Taq + Q-solution:
MAF_ex 1.5R   AGAACTAGCAAGCCCACACC                 Predenat: 98°C, 5 min; denat: 96°C,
                                                   30 s; ann. 53.5°C, 30 s, ext. 72°C,
                                                   40 cycles

MAF_ex 1.3F   AACTGGCAATGAGCAACTCC       548 bp    Qiagen HotStart Taq + Q-solution:
MAF_ex 1.3R   GTGGTGGTGGTGGTGGTAGT                 Predenat: 98°C, 5 min; denat. 96°C,
                                                   30 s; ann. 60°C, 30 s, ext. 72°C,
                                                   40 cycles

MAF_ex1.2f2   GAGCGAGGGAGCACATTG         352 bp    Qiagen HotStart Taq + Q-solution:
MAF_ex 1.2r   CCGGTTCCTTTTTCACTTCA                 Predenat: 98°C, 5 min; denat. 96°C,
                                                   30 s; ann. 60°C, 30 s, ext. 72°C,
                                                   40 cycles

MAF_ex1.4f3   CCGCACTACCACCACCAC         432 bp    Qiagen HotStart Taq + Q-solution:
MAF_ex 1.4R   CTGGTTCTTCTCCGACTCCA                 Predenat: 98°C, 5 min; denat. 96°C,
                                                   30 s; ann. 60°C, 30 s, ext. 72°C,
                                                   40 cycles

MAF_ex2f      AAATCCTGAGTAAGTGCCATTCA    575 bp    New England Biolab Taq:
MAF_ex2r      GTTGCATTCCGGGAAACTT                  Predenat: 95°C, 5 min; denat. 95°C,
                                                   30 s; ann. 60°C, 30 s, ext. 72°C,
                                                   40 cycles

Hansen, Mol Vis 2007; 13:2019-2022 <http://www.molvis.org/molvis/v13/a227/>
©2007 Molecular Vision <http://www.molvis.org/molvis/>
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