Table 1 of
McGowan, Mol Vis 2007;
13:1984-2000.Table 1 of
McGowan, Mol Vis 2007;
13:1984-2000.
Table 1. Primary antibodies
Below is a list of primary antibodies used for protein detection together with their animal sources, commercial sources and concentrations. Working concentrations of the corresponding secondary antibodies are listed here for convenience. Secondary antibody descriptions are listed in Table 2. All antibodies were used as described in the Methods section. The asterisk indicates Abcam, Cambridge, MA and the sharp (hash mark) indicates R&D Systems, Minneapolis, MN.
Concentration (μg/ml)
-----------------------------------------
Source/ Cross sections Flat mounts
catalog ------------------- -------------------
Antibody Description ID Primary Secondary Primary Secondary
---------- ------------------ ------- ------- --------- ------- ---------
Nestin mouse, monoclonal Abcam* 0.1 0.5 0.03 0.2
ab6320
Oct-3/4 rat, monoclonal R&D# 0.5 1.0 0.1 0.2
MAB1759
Pax-6 rabbit, polyclonal Abcam* 1.3 0.2 0.2 0.2
ab5790
Sox-2 mouse, monoclonal R&D# 0.5 1.0 0.5 0.2
MAB2018
Telomerase rabbit, monoclonal Abcam* 0.75 1.3 0.6 0.4
ab32020
Wnt-1 rabbit, polyclonal Abcam* 0.25 1.3 0.07 0.2
ab15251
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