Figure 3. Nestin in the unwounded cornea

Brightfield image (A) and corresponding fluorescence image (B) shows the presence of nestin in the trabecular meshwork and the transition zone of a cross section of the unwounded cornea. Nestin is not present in the corneal endothelium although there is some autofluorescence indicated at the border of the stroma and Descemet's membrane (D); corresponding brightfield image (C). Sections are from a 20-year-old male. Flat mount of an unwounded cornea shows nestin in the transition zone with an arrow indicating the same cells in the corresponding panels (E, F). Flat mount cornea is from a 64-year-old female. Controls showed no reaction (not shown).