Figure 11. Double labeling: nestin and Pax-6

The wounded cornea is from a 38-year-old male. Pax-6 (red) was present in the endothelium, transition zone, and the interior border cells of Schlemm's canal, and nestin (bright green) is present in the trabecular meshwork and at the transition zone (A). Double labeling in the unwounded cornea of 29-year-old male shows no expression of Pax-6 (B). Bright green indicates nestin; red indicates Pax-6; and blue indicates cell nuclei from Hoechst stain. Control slide showed no reaction (not shown).