Table 1 of
Takacs, Mol Vis 2007;
13:1976-1983.Table 1 of
Takacs, Mol Vis 2007;
13:1976-1983.
Table 1. Primers used for sequencing exons of TGFBI
Primers have been optimized for PCR amplification of the 17 exons of TGFBI from genomic DNA. In addition to entire exons, short segments of their flanking introns were also covered. Exon 17 was sequenced in three overlapping segments.
Annealing product
temperature length
Exon primer Sequence (°C) (bp)
---- -------- ------------------------ ----------- -------
1 forward: 5-CCCTCCCGCTCGCAGCTTAC-3 55 424
reverse: 5-CCCTCTCTCCTTCCACCGCG-3
2 forward: 5-GACCCAAGGAGGCAAACACG-3 55 232
reverse: 5-CCAGCGTGCATACAGCTTGG-3
3 forward: 5-CTCTTCCTTGGCTGTGGAGG-3 55 228
reverse: 5-TCCTCTCTCCCACCATTCCC-3
4 forward: 5-CCCCAGAGGCCATCCCTCCT-3 58 226
reverse: 5-CCGGGCAGACGGAGGTCATC-3
5 forward: 5-TCACGAGGGCTGAGAACATG-3 55 341
reverse: 5-CAGAAACCAGCCCACACATG-3
6 forward: 5-GCTTTGGGACTATGCCTCTG-3 58 260
reverse: 5-CTCTTGGGAGGCAATGTGTC-3
7 forward: 5-GAGCTTGGGTTTGGCTTCTG-3 55 350
reverse: 5-AGCAGGTCTTGGGCTCAATC-3
8 forward: 5-GCTGCAAGTGGTCCCTGAGG-3 60 407
reverse: 5-AGCTGTGAGCTGGGTCCAGG-3
9 forward: 5-TGCTGATGTGTGTCATGCCC-3 55 426
reverse: 5-CCCAAAGGCATTCTCTGCAC-3
10 forward: 5-CCAAACTCAAGGAGGGATGG-3 58 373
reverse: 5-CCTTCCTTGTCAGCAACCAG-3
11 forward: 5-CTGAACAAATCAGGAGGCCC-3 55 288
reverse: 5-CTTGACAAATAGCTGGGGCC-3
12 forward: 5-TGTGCATTCCAGTGGCCTGG-3 55 339
reverse: 5-GCCAACTGTTTGCTGCTGGG-3
13 forward: 5-GTGCTTATTCCCTGGGCAGG-3 55 317
reverse: 5-ATATGTCCTGGAGCCCTGCC-3
14 forward: 5-TCGAGATCATGCCACTGCAC-3 55 344
reverse: 5-TCTCCACCAACTGCCACATG-3
15 forward: 5-AGCCAGAAAGCCCACCAGTG-3 58 275
reverse: 5-AGCAGCCAAGGAAGACAGGG-3
16 forward: 5-TCTGCCAAGGCCACCTGAGG-3 58 278
reverse: 5-CAGCAGATGGCAGGCTTGGG-3
17/A forward: 5-AGAGCATGGCAGAAGGAGGC-3 55 306
reverse: 5-CCCACATGCACAAGGCTCAC-3
17/B forward: 5-TACAGGAGGAATGCACCACG-3 55 376
reverse: 5-AAACTGTGCCAGGACTTCCC-3
17/C forward: 5-GACTGCCTATGCCAAGTCCC-3 58 364
reverse: 5-AGGTGTGGAGGAGCATGACG-3
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