Table 1 of
Jin, Mol Vis 2007;
13:1953-1961.Table 1 of
Jin, Mol Vis 2007;
13:1953-1961.
Table 1. Primers for real-time polymerase chain reaction
Specific primers for TLR1-10 mRNAs were designed using Prime 3 software, possibly to be 1-intron spanning.
Amplicon
Target size
gene Locus Forward sequence(5'-3') Reverse sequence (5'-3') (bp)
------ -------- ----------------------- ------------------------ --------
TLR1 NM003263 GCCCAAGGAAAAGAGCAAAC AAGCAGCAATATCAACAGGAG 135
TLR2 NM003264 TCTCCCATTTCCGTCTTTTT GGTCTTGGTGTTCATTATCTTC 125
TLR3 NM003265 TAAACTGAACCATGCACTCT TATGACGAAAGGCACCTATC 101
TLR4 NM003266 GAAGCTGGTGGCTGTGGA GATGTAGAACCCGCAAG 213
TLR5 NM003268 TTGCTCAAACACCTGGACAC CTGCTCACAAGACAAACGAT 148
TLR6 NM006068 GTGCCATTACGAACTCTA TTGTTGGGAATGCTGTT 109
TLR7 NM016562 CTGACCACTGTCCCTGAG AACCCACCAGACAAACCA 263
TLR8 NM016610 AACATCAGCAAGACCCAT GACTCCTTCATTCTCCCT 64
TLR9 NM017442 CGCCAACGCCCTCAAGACA GGCGCTTACATCTAGTATTTGC 79
TLR10 NM030956 CTCCCAACTTTGTCCAGAAT GGTGGGAATGCAATAGAAT 132
GAPDH CCCCACACACATGCACTTACC TTGCCAAGTTGCCTGTCCTT 100
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