Figure 1. Tissue specific expression of ovalbumin

Tissues were removed from B6 or B6.TRP-1-OVA mice and RNA extracted using RNAzol^TM. The RNA was treated with DNase I and then phenol/chloroform extracted. Next, first strand cDNA was synthesized and used as a template for PCR amplification. A first round of PCR was performed using OVA primers 96-115 and 656-675. Next, a second round of PCR was performed using nested OVA primers 109-130 and 574-593. Results were visualized on a 2% agarose gel treated with ethidium bromide; the buffer control (buff) which contained no RNA served as a negative control and the plasmid (+) and RNA from E.G7-OVA served as positive controls. The GAPDH housekeeping gene was positive for all samples (not shown). All samples were run on the same gel but extraneous lanes were digitally removed from the image, as shown by the gaps in the gel.