Figure 2. Proteins associated with CD81

Cultured rat retinal pigment epithelium (RPE) cells were solubilized in 1% Brij 97 and immunoprecipitated with mouse anti-rat CD81 antibody. The sample of proteins was then separated by SDS PAGE, transferred to nitrocellulose, and probed with antibodies directed against CD81 (lane A), EBP50 (lane B), Ezrin (lane C), and Sap97 (lane D). Note that CD81 coimmunoprecipitated with EBP50, Ezrin, and Sap97. In control experiments, antibodies directed against the transferrin receptor (CD71) were used to immunoprecipitate proteins from cultured RPE. When these samples were probed for EBP50 (lane E) or Sap97 (lane F), these two PDZ proteins were not observed. Molecular weight markers are indicated to the left in kDa.