Table 1 of
Iannaccone, Mol Vis 2007;
13:1856-1861.Table 1 of
Iannaccone, Mol Vis 2007;
13:1856-1861.
Table 1. Polymerase chain reaction primers and conditions used to amplify genomic segments from GRP143
Polymerase chain reaction primers and conditions used to exons and intron/exon junction from the OA1 gene. OA1 exons specific primer sequences and annealing conditions are provided.
Annealing
Target Primer name Primer sequence (5'-3') T (°C)
---------- ----------- -------------------------------- ---------
OA1 exon 1 OA1x1F3 GAGCCTGGCTCTACTGCAGGCGCT 64
5' end OA1x1R3 CCTTCCACGCGCTCTGCCTGGGCA
OA1 exon 1 OA1x1F4b AGCCGCGCAGCTCGTGCTGAGCTTCCAGCC 68
3' end OA1x1R4 CCGCGGGTTGGAATCTGATCAGCGCCTGGG
OA1 exon 2 OA1x2F1 TCATTTTTCCAAAGCAAGAAGTCAGC 66
OA1x2R1 GCAGGACGTGAGAACCTGCATT
OA1 exon 3 OA1x3F1 GTCTACCCTGCCGTCTCAAGGATG 66
OA1x3R1 CGCTCAGTGCCATCTCTTATCTTCC
OA1 exon 4 OA1x4F1 GTTCCAGGCAGGCCTCTGTGC 68
OA1x4R1 GGCTCATGTATTCCCTGCAAGACAAC
OA1 exon 5 OA1x5F1 TTTCCCTTTTTGTTCTCATCCTCTTA 63
OA1x5R1 AGGACAACATGTGTCACTGTCTGAG
OA1 exon 6 OA1x6F1 ACCTGCTTCCATTGCCTTCTCTGTC 68
OA1x6R1 CTTCCCTTTGGAACTTCTGGTCACG
OA1 exon 7 OA1x7F1 GAAATTCTTCTCTGACTCTCCAGCATT 63
OA1x7R1 TGACAGAGTGAGACCTTGTCTCTGA
OA1 exon 8 OA1x8F1 ATGGTCCCTTCCAAGCGAGTCC 68
OA1x8R1 TCACATGAGAGGTGCTGCTGAACAC
OA1 exon 9 OA1x9F1 TGAAAAACTCCATGCACTGAATACT 61
OA1x9R1 TGCATAACTGTACATGTATTTATTTTCTTTTG
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