Table 3 of
Redmond, Mol Vis 2007;
13:1813-1821.Table 3 of
Redmond, Mol Vis 2007;
13:1813-1821.
Table 3. Effect of R/K substitution at aa446 of RPE65
Interchange of lysine for arginine at aa446 of dog RPE65 increases isomerase activity, while interchanging arginine for lysine at aa446 of mouse RPE65 reduces isomerase activity. 1Production of 11-cis retinol. 2RPE65 immunoreactive protein. 3Calculated as percentage of wildtype value.
Isomerase1 RPE652
Name activity3 S.D. Protein3 S.D. N
------------ ---------- ---- -------- ---- -
dWT/R446K 119.9 3.6 131.4 12.5 3
dL450M 73.9 4.9 85.4 12.4 3
dL450M/R446K 93.9 8.7 90.1 18.2 3
mWT/K446R 81.3 5.7 102.6 23.3 3
ml450M 40.4 3.8 71.1 8.5 3
ml450M/K446R 37.7 8.3 91.4 14.1 3
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