Redmond, Mol Vis 2007; 13:1813-1821. Table 1.

Table 1 of Redmond, Mol Vis 2007; 13:1813-1821.

Table 1. Oligonucleotide primers used for site-directed mutagenesis of RPE65

Prefixes D and M refer to oligonucleotides synthesized for dog and mouse sequences, respectively; suffixes F and R refer to forward and reverse primers, respectively.

     Name                         Primer
--------------   ----------------------------------------
D-L450M-F        5'-GACAGGCTCTGCAAGATGAACGTCAAGACTAAAG-3'
D-L450M-R        5'-CTTTAGTCTTGACGTTCATCTTGCAGAGCCTGTC-3'
M-L450M-F        5'-GACAAGCTCTGTAAGATGAACGTCAAAACT-3'
M-L450M-R        5'-AGTTTTGACGTTCATCTTACAGAGCTTGTC-3'
D-V459M-F        5'-ACTAAAGAAACGTGGATGTGGCAAGAGCCCGAC-3'
D-V459M-R        5'-GTCGGGCTCTTGCCACATCCACGTTTCTTTAGT-3'
M-M459V-F        5'-ACTAAAGAAATCTGGGTGTGGCAAGAGCCA-3'
M-M459V-R        5'-TGGCTCTTGCCACACCCAGATTTCTTTAGT-3'
D-T457I-F        5'-GTCAAGACTAAAGAAATCTGGGTATGGCAAGAG-3'
D-T457I-R        5'-CTCTTGCCATACCCAGATTTCTTTAGTCTTGAC-3'
M-I457T-F        5'-GTCAAAACTAAAGAAACGTGGATGTGGCAAGAG-3'
M-I457T-R        5'-CTCTTGCCACATCCACGTTTCTTTAGTTTTGAC-3'
D-R446K/L450-F   5'-CACTTCGTTCCGGACAAGCTCTGCAAGCTGAAC-3'
D-R446K/L450-R   5'-GTTCAGCTTGCAGAGCTTGTCCGGAACGAAGTG-3'
M-R446K/L450-F   5'-CACTTTGTTCCTGACAGGCTCTGTAAGCTGAAC-3'
M-R446K/L450-R   5'-GTTCAGCTTACAGAGCCTGTCAGGAACAAAGTG-3'
D-R446K/M450-F   5'-CACTTCGTTCCGGACAAGCTCTGCAAGATGAAC-3'
D-R446K/M450-R   5'-GTTCATCTTGCAGAGCTTGTCCGGAACGAAGTG-3'
M-R446K/M450-F   5'-CACTTTGTTCCTGACAGGCTCTGTAAGATGAAC-3'
M-R446K/M450-R   5'-GTTCATCTTACAGAGCCTGTCAGGAACAAAGTG-3'
D-T457N-F        5'-GTCAAGACTAAAGAAAACTGGGTATGGCAAGAG-3'
D-T457N-R        3'-CTTTAGTCTTGACGTTCCGCTTGCAGAGCCTGTC-3'
D-L450R-F        5'-GACAGGCTCTGCAAGCGGAACGTCAAGACTAAAG-3'
D-L450R-R        5'-CTTTAGTCTTGACGTTCCGCTTGCAGAGCCTGTC-3'

Redmond, Mol Vis 2007; 13:1813-1821 <http://www.molvis.org/molvis/v13/a202/>