Figure 4. Presence of CD4 and CD8 mRNA in the corneas of naive mice

Corneas or spleens from eight BALB/c or C57BL/6 mice and four SCID mice were harvested. Total RNA was isolated and TaqMan RT-PCR was performed as described in Methods using CD4 and CD8 primers. Following cDNA synthesis, 10 μl of cDNA for each group was run on individual lanes of a 0.9% agarose. The data shown are representative of one of four experiments.