Figure 2. Detection of CD4/CD3-postitive and CD8/CD3-positive T cells in immunostained sections of the cornea of naive mice

Representative corneal sections from naive BALB/c mice stained with anti-CD4 and anti-CD3 or anti-CD8 and anti-CD3 mAbs as described in Methods are shown. Panels: Sections of cornea from naive BALB/c mice were stained with anti-CD4 (CD4), anti-CD3 (CD3), or merged (CD4+CD3). Arrows show CD4⁺, CD3⁺, and CD4/CD3 double-positive T cells in the stromal cell layer of the cornea. Sections of the cornea from naive BALB/c mice were stained with anti-CD8 (CD8), anti-CD3 (CD3), or merged (CD8/CD3). The arrows show CD8⁺, CD3⁺, and CD8/CD3 double-positive T cells in the stromal cell layer of the cornea.