Table 1 of
Hogewind, Mol Vis 2007;
13:1793-1801.Table 1 of
Hogewind, Mol Vis 2007;
13:1793-1801.
Table 1. Primer sequences and polymerase chain reaction conditions for amplifying three exons of MYOC
For sequencing of exon 1, two nested primers were used: 3F (5'-AGT GGC CGA TGC CAG TAT AC-3') and 3R (5'-CTG GTC CAA GGT CAA TTG GT-3'). A similar method was used to sequence exon 3, the two nester primers were: 10F (5'- ATA CTG CCT AGG CCA CTG GA-3') and 10R (5'- CAA TGT CCG TGT AGC CAC C-3'). Exon 2 was sequenced with the same primers used for PCR. All the primers were obtained from the literature [37].
Annealing Amplicon
temperature Mg2+ size
Exon Forward primer (5'-3') Reverse primer (5'-3') (°C) (mM) (bp)
---- ------------------------- ------------------------ ----------- ---- --------
1 1F-GGCTGGCTCCCCAGTATATA 6R-CTGCTGAACTCAGAGTCCCC 58 2.0 782
2 7F-AACATAGTCAATCCTTGGGCC 7R-GAATAAAGACCACGTGGGCAC 52 2.0 237
3 8F-TTATGGATTAAGTGGTGCTTCG 13R-AGCATCTCCTTCTGCCATTG 58 2.5 872
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