Table 2 of
Dekomien, Mol Vis 2007;
13:174-180.Table 2 of
Dekomien, Mol Vis 2007;
13:174-180.
Table 2. Primers for amplification of exonic regions of CACNA2D3, HT017, and WNT5A including exon/intron boundaries
The intronic primer sequence for amplification of corresponding exons and 50 intronic bases on either end of the exons and the product sizes are shown.
Size
Gene Exon Sequence (5'-3') (bp)
-------- ------ -------------------------------- ----
CACNA2D3 1F GGTGCTGGCAGTTTCTACCC 268
1R ATCGCTTCCTGCCACCAC
2F AAGAGAGGCAGTCCTATTTATTCCTTG 329
2R GAACAACTGAGTCAACTTCACTCTTTG
3F AAGGCTCCAGGTCATAATGCAC 261
3R CTTACCATTTCTTGTTTGGCAGC
4F GACACCCTTACCTGGCTTTGC 355
4R TGTCCCCCATACCCTCTGTTC
5F CTCATGCTGCTATTGCCAGC 326
5R TCATTTTCCCCTATTGGGAGG
6+7F CAGGGCTGGTCATTGCATG 546
6+7R AGGCGGGACATCTAATGCTG
8F GGTACCCCTCAAATGATTAATTGTG 201
8R TAGGTTTGTATCATGACCCTTGATG
9F CCATTCCAGTCTGAGACTCGTTG 311
9R GCCAAGGCTAACCATAATGCTG
10F GGGAAATGCTTATCGTGTAGGTTC 422
10R AGAGCCATAGTATTCGGTTTGGTC
11F CCTACAGATGGGAAGCCTGG 277
11R CTCTTAACTATCCCAACTTGCGC
12F TGAGAGGTGGGTTTATGGTTGAC 394
12R GGTTAACACGGTCTTCTGGAATTAAC
13F TTGCCAGTGGTGACCAAATG 208
13R GTACTGAATGCACAAAATCATGGAG
14F ACTGGTCTGTTCCCAATGGC 266
14R CACCAGAGCTTGAGTCAGAGAATG
15F AAACTTTGAGAAGGCACCAGATG 248
15R AGGTATCTTGGACGCTTAGTCCTG
16F TGAATTCACCAGGGAGCCAG 221
16R AAACGCTGCTGCAAAAGTGTC
17F GCTTCTCTCCAATATCCCTCCAC 279
17R TGGCCTCCAGGTACAGAACTG
18+19F AGCATGCTGACTTGGTGCC 693
18+19R CACTCTTTCCAGCCTTTTGGC
20F AAACCAACACCAACTATAATCAGTGG 310
20R TCTTTCATTAATCCAATGACTGGC
21+22F GGGAGATGCAAGTGAGGAGG 637
21+22R GCTGGATTTGATGGGACCG
23F AGAACTGTTCCATATTTTCAGCTGG 406
23R TCGAGCTCTTGTGGTTTGGAG
24F TTGCATTCTGTGCTTGGTGC 359
24R ATCAAGAAACGCAAGCCTCC
25F TCTCCACACTTTCGCCAACAC 484
25R GGCCAATATTCAACACTGCCTC
26F CACAGCAGCTATGGCGTCAC 410
26R ACCAGCTGTCAGAATCTTGAATTG
27F TGCTTGCATCAGCTTCTTGC 251
27R GCACTTTTAGGCTCCGGGTAC
28F TAAGTGGCAATCATAGCAGATGC 232
28R TCAGGAGCAATCTGTCAGACAAG
29F CCATGGCCTTAACTCCTAGAGC 381
29R GGCTGTGACATTTTTAGAGGGATAG
30F TCCTGATTCTTCTCTGGTGCTTG 231
30R GGCTTGCTTTATTTGACCTTGG
31F GACTTTGCATGTTCCTGGTTTG 304
31R CACAGATAAAGATATGCTCACGCTC
32F GGTATCCAACCATGTTCTTCTTGTAG 373
32R TGGTCTCAGCTTTCAATATTTCGAG
33F GGGTAACACAACCAACCATACTGTAC 290
33R TGTATGTTTGTCCATCCTTGGTG
34F GAGCGGAACTGGGTTCTGAG 352
34R TCTAGCGAAGCCAAATGATGC
35+36F GGGAGTTTTCCTCAGGATCTTTG 551
35+36R ATGAGACTCTCATGGTGAATCTGG
37F AAGACTTGATTCCTACTTTGGAGAGC 630
37R AAGTCTCTGCCAACAACCATCC
HT017 1F GCGTTGAAGAAAAGCACAAGC 209
1R AGTCCCTTCCTCCCACGG
2aF ATTCCTTGTGACTTCAGAGGGAC 487
2aR GGAGCTTGATTTGTCATCAAACA
2bF GCAGACTTTTCCACTCCCTCC 319
2bR CCTGCGTGTTACCAGCCTAGT
3F GCTGTATATATTCCATCTGCCTGAG 605
3R GTGAATGCATCAACCCTACTCATATAC
WNT5A 1F GTAAAGTCTTTTGCACAATCACGC 468
1R AAAAAGTGGCGAGCGTCG
2F AACTCAACGGAGGAGAAGCG 302
2R AATAAAACAAAGCATATGTACTTAGAAGGAAC
3F ACTTTGTCATGAGGACAAGCAGG 440
3R TCTTCAGGAGAACACTTGATCCG
4F GGGTCAGAGTGGAGACGCC 503
4R TTGTCAGGCAGCATCAGGC
5F GGAGCGGAGCTTTGGTAACC 599
5R AAATAAGTGGGTCCTGGGAGC
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