Table 1 of
Li, Mol Vis 2007;
13:1758-1768.Table 1 of
Li, Mol Vis 2007;
13:1758-1768.
Table 1. Summary and analysis of the fast protein liquid chromatography file and chaperone-like activity of D51- and wt-αA-crystallin and mutants.
The asterisk indicates that the subunit molecular weight is calculated based on its protein sequence using program ProtParam. The double asterisk indicates that the chaperone-like activities of all D51 mutants are not as good as that of the wt-αA-crystallin and its two mutants. The + or - in this column indicates chaperone activity level relative to D51-αA-crystallin.
Width MW at
Percent at the the
of half peak Subunit Number
total peak point MW of Chaperone-like
Mutant name Peak name area height (kDa) (Da)* subunits activity**
----------- ---------------------- ------- ------ ----- ------- -------- --------------
D51-alphaA Primary peak 99 0.78 60.9 15154 4.0 A
Second peak, shoulder 0.90 14.8 15154 1.0
D51-ITV Primary peak 89 0.84 48.5 15158 3.2 A-
Second, large shoulder 0.66 10.7 15158 0.7
D51-IGV Primary peak 100 0.79 57.0 15114 3.8 A+
D51-GGG Primary peak 84 0.70 51.2 15015 3.4 A-
Second, large shoulder 0.55 10.5 15015 0.7
D51-GPG Primary peak 100 0.69 65.4 15055 4.3 A+
D51-TPT Primary peak 84 0.96 63.9 15134 4.2 A
Second, shoulder 1.16 15.8 15134 1.0
Wt-alphaA Primary peak 75 1.56 54.0 19790 27.3 Almost
identical
Second peak 25 1.82 84.3 19790 4.2
Third, shoulder 1.24 56.4 19790 2.8
Wt-ITV Primary peak 63 2.10 413.3 19794 20.9
Second peak 37 1.62 87.8 19794 4.4
Third shoulder 1.24 51.2 19794 2.6
Wt-IGV Primary peak 65 2.12 608.9 19750 30.8
Second peak 35 1.42 86.8 19750 4.4
Third shoulder 0.98 51.4 19750 2.6
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