Table 1 of Li, Mol Vis 2007; 13:1758-1768.


Table 1. Summary and analysis of the fast protein liquid chromatography file and chaperone-like activity of D51- and wt-αA-crystallin and mutants.

The asterisk indicates that the subunit molecular weight is calculated based on its protein sequence using program ProtParam. The double asterisk indicates that the chaperone-like activities of all D51 mutants are not as good as that of the wt-αA-crystallin and its two mutants. The + or - in this column indicates chaperone activity level relative to D51-αA-crystallin.

                                                 Width    MW at
                                       Percent   at the    the
                                         of       half    peak    Subunit    Number
                                        total     peak    point     MW         of      Chaperone-like
Mutant name         Peak name           area     height   (kDa)    (Da)*    subunits     activity**
-----------   ----------------------   -------   ------   -----   -------   --------   --------------
D51-alphaA    Primary peak                99      0.78     60.9    15154       4.0           A
              Second peak, shoulder               0.90     14.8    15154       1.0
D51-ITV       Primary peak                89      0.84     48.5    15158       3.2           A-
              Second, large shoulder              0.66     10.7    15158       0.7
D51-IGV       Primary peak               100      0.79     57.0    15114       3.8           A+
D51-GGG       Primary peak                84      0.70     51.2    15015       3.4           A-
              Second, large shoulder              0.55     10.5    15015       0.7
D51-GPG       Primary peak               100      0.69     65.4    15055       4.3           A+
D51-TPT       Primary peak                84      0.96     63.9    15134       4.2           A
              Second, shoulder                    1.16     15.8    15134       1.0
Wt-alphaA     Primary peak                75      1.56     54.0    19790      27.3         Almost
                                                                                         identical
              Second peak                 25      1.82     84.3    19790       4.2
              Third, shoulder                     1.24     56.4    19790       2.8
Wt-ITV        Primary peak                63      2.10    413.3    19794      20.9
              Second peak                 37      1.62     87.8    19794       4.4
              Third shoulder                      1.24     51.2    19794       2.6
Wt-IGV        Primary peak                65      2.12    608.9    19750      30.8
              Second peak                 35      1.42     86.8    19750       4.4
              Third shoulder                      0.98     51.4    19750       2.6

Li, Mol Vis 2007; 13:1758-1768 <http://www.molvis.org/molvis/v13/a196/>
©2007 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535