Figure 3. Size exclusion chromatograms for D51-αA-crystallin mutants

Oligomeric sizes were estimated using a Superose 12 10/300 GL column calibrated with molecular weight standards and run with 0.1 M NaCl Tris buffer, pH 8.0. A-F are the FPLC profiles for D51-αA, D51-ITV, D51-IGV, D51-GGG, D51-GPG, and D51-TPT, respectively. The y-axis value, OD360, was normalized by Origin 6.1 so that the area under the curve of each mutant was the same. The positions of proteins used for standard molecular masses are indicated at the top of the diagram. The solid black square indicates β-amylase (220 kDa), a triangle indicates alcohol dehydrogenase (150 kDa), a dot indicates bovine serum albumen (66 kDa), a diamond indicates carbonic anhydrase (29 kDa), and a star indicates cytochrome C (12.4 kDa).