Figure 9. Paraptosis in triamcinolone treated-ARPE-19 cells

A: Effect of Alix overexpression on TA induced cell death. ARPE 19 cells were nucleofected with Alix-WT or with its C terminal moiety. Controls were run with untransfected cells or in cells transfected with the PCI empty vector. 48 h after transfection cells were treated with TA and cell survival was measured with the 3-(4,5-dimethylthiazol-2-yl)-2,5 phenyltetrazolium bromide test method. Alix-CT has no effect on cell survival but Alix-WT protects cells form death. * indicates a p<0.05. B: Western-blot using anti-PJNK (upper panel) or anti-JNK-1 (lower panel) was assayed in 0.1 mg/ml (in 1% ethanol) TA treated (TA) or control (C, 1% ethanol) ARPE-19 cells. An increase of P-JNK-2 is seen in TA treated cells, while no JNK-1 over activation is observed in TA-treated cells compared to control cells.