Figure 7. Markers of autophagy in triamcinolone treated ARPE-19 cells

A-C: Monodansylcadaverine staining in control (A) and 0.1 mg/ml (in 1% ethanol) TA-treated ARPE-19 cells (B and C). Small monodansylcadaverine positive (B) vesicles (white arrowheads), and larger vesicles not stained with monodansylcadaverine (C) are observed in TA-treated cells. D: An anti-LC3 immunostaining in control (A) and TA-treated ARPE-19 cells (B), and in cells treated with cells an amino acid deplete medium (AA dep; C). LC3 is evenly distributed in control and TA-treated cells. In cells treated with AAdep, LC3 is concentrated in autophagic vesicles. Scale bar represents 20 μm.