Figure 6 of
Valamanesh, Mol Vis 2007;
13:1746-1757.
Figure 6. No caspase-independent markers of apoptosis in triamcinolone treated ARPE-19 cells
A- G: Triamcinolone-treated (0.1 mg/ml in 1% ethanol) or control ARPE 19 cells were stained with DAPI or immunostained with anti-AIF or anti LEI/L-DNase II. No nuclear translocation of these apoptosis markers was seen, neither in cells with normal morphology, nor in cells with an altered nuclear morphology (A, white arrows). H-M: Induction of apoptosis with HMA was used as positive control and shows nuclear translocation of both LEI and AIF, both markers of caspase independent apoptosis. Sacle bar represents 20 μm.