Figure 4. Absence of activation of caspase 3 in ARPE-19 cells

Subconfluent ARPE 19 cells were incubated for 72 h in the presence 0.1 mg/ml triamcinolone (in 1% ethanol). Protein extracts were separated in 4-10% PAGE, transferred to nitrocellulose and revealed with anti-caspase 3. No activation of caspases 3 is seen in TA-treated cells. Apoptosis induced in HL-60 with etoposide (50 μM for 24 h) was used as positive control.