Table 1 of
Abouzeid, Mol Vis 2007;
13:1740-1745.
Table 1. Sequence of primers and polymerase chain reaction conditions
Forward (F) and reverse (R) primers used to amplify the promoter region and the various RB1 exons. Expected size and polymerase chain reaction annealing temperatures are provided.
Amplicon Annealing Region Primer Sequence (5'-3') (bp) (°C) ------------- ------ -------------------------- -------- --------- Promoter PromF CTGGACCCACGCCAGGTTTC 340 61 PromR GTTTTGGGCGGCATGACGCCTT RB exon 1 1F CCGGTTTTTCTCAGGGGACGTTG 340 56.4 1R TTGGCCCCGCCCTACGCACAC RB exon 2 2F CTATTGAAACAAGTATGTACTG 331 54.3 2R GGGTAATGGAATTATTATTAGC RB exon 3 3F CAGTTTTAACATAGTATCCAG 281 52.8 3R AGCATTTCTCACTAATTCAC RB exon 4 4F GTAGTGATTTGATGTAGAGC 305 55 4R CCCAGAATCTAATTGTGAAC RB exon 5 5F GCATGAGAAAACTACTATGAC 194 54.3 5R CTAACCCTAACTATCAAGATG RB exon 6 6F CACCCAAAAGATATATCTGG 222 54.3 6R ATTTAGTCCAAAGGAATGCC RB exon 7 7F CCTGCGATTTTCTCTCATAC 256 55 7R ATGTTTGGTACCCACTAGAC RB exon 8 8F AGTAGTAGAATGTTACCAAG 380 50.8 8R TACTGCAAAAGAGTTAGCAC RB exon 9 9F TGCATTGTTCAAGAGTCAAG 222 56 9R AGTTAGACAATTATCCTCCC RB exon 10 10F TCTTTAATGAAATCTGTGCC 291 56 10R GATATCTAAAGGTCACTAAG RB exon 11 11F GAGACAACAGAAGCATTATAC 245 54.2 11R CGTGAACAAATCTGAAACAC RB exon 12 12F GGCAGTGTATTTGAAGATAC 310 52.5 12R AACTACATGTTAGATAGGAG RB exon 13 13F CTTATGTTCAGTAGTTGTGG 342 54.3 13R TATACGAACTGGAAAGATGC RB exon 14 14F GTGATTTTCTAAAATAGCAGG 212 58.9 14R TGCCTTGACCTCCTGATCTG RB exon 15+16 15/16F CAATGCTGACACAAATAAGG 366 55 15/16R AGCATTCCTTCTCCTTAACC RB exon 17 17F AAAAATACCTAGCTCAAGGG 339 56 17R TGTTAAGAAACACCTCTCAC RB exon 18 18F TGTACCTGGGAAAATTATGC 340 56.4 18R CTTTATTTGGGTCATGTACC RB exon 19 19F ATAATCTGTGATTCTTAGCC 273 56 19R AAGAAACATGATTTGAACCC RB exon 20 20F AAAGAGTGGTAGAAAAGAGG 335 56.4 20R CAGTTAACAAGTAAGTAGGG RB exon 21 21F AAACCTTTCTTTTTTGAGGC 328 54 21R TACATAATAAGGTCAGACAG RB exon 22 22F TAATATGTGCTTCTTACCAGTC 313 56 22R TTTAATGTTTTGGTGGACCC RB exon 23 23F ATCTAATGTAATGGGTCCAC 287 54.2 23R CTTGGATCAAAATAATCCCC RB exon 24 24F GAATATAGTTTGTCAGTGGTTC 273 52 53 24R GTGTTTGAATAACTGCATTTGG RB exon 25 25F GGTTGCTAACTATGAAACAC 297 54.2 55 25R AGAAATTGGTATAAGCCAGG RB exon 26 26F AGTAAGTCATCGAAAGCATC 209 52.8 26R AACGAAAAGACTTCTTGCAG RB exon 27 27F CGCCATCAGTTTGACATGAG 237 54.2 |