Table 1 of Abouzeid, Mol Vis 2007; 13:1740-1745.


Table 1. Sequence of primers and polymerase chain reaction conditions

Forward (F) and reverse (R) primers used to amplify the promoter region and the various RB1 exons. Expected size and polymerase chain reaction annealing temperatures are provided.

                                                      Amplicon   Annealing
   Region       Primer       Sequence (5'-3')           (bp)       (°C)
-------------   ------   --------------------------   --------   ---------
Promoter        PromF    CTGGACCCACGCCAGGTTTC           340        61
                PromR    GTTTTGGGCGGCATGACGCCTT
RB exon 1       1F       CCGGTTTTTCTCAGGGGACGTTG        340        56.4
                1R       TTGGCCCCGCCCTACGCACAC
RB exon 2       2F       CTATTGAAACAAGTATGTACTG         331        54.3
                2R       GGGTAATGGAATTATTATTAGC
RB exon 3       3F       CAGTTTTAACATAGTATCCAG          281        52.8
                3R       AGCATTTCTCACTAATTCAC
RB exon 4       4F       GTAGTGATTTGATGTAGAGC           305        55
                4R       CCCAGAATCTAATTGTGAAC
RB exon 5       5F       GCATGAGAAAACTACTATGAC          194        54.3
                5R       CTAACCCTAACTATCAAGATG
RB exon 6       6F       CACCCAAAAGATATATCTGG           222        54.3
                6R       ATTTAGTCCAAAGGAATGCC
RB exon 7       7F       CCTGCGATTTTCTCTCATAC           256        55
                7R       ATGTTTGGTACCCACTAGAC
RB exon 8       8F       AGTAGTAGAATGTTACCAAG           380        50.8
                8R       TACTGCAAAAGAGTTAGCAC
RB exon 9       9F       TGCATTGTTCAAGAGTCAAG           222        56
                9R       AGTTAGACAATTATCCTCCC
RB exon 10      10F      TCTTTAATGAAATCTGTGCC           291        56
                10R      GATATCTAAAGGTCACTAAG
RB exon 11      11F      GAGACAACAGAAGCATTATAC          245        54.2
                11R      CGTGAACAAATCTGAAACAC
RB exon 12      12F      GGCAGTGTATTTGAAGATAC           310        52.5
                12R      AACTACATGTTAGATAGGAG
RB exon 13      13F      CTTATGTTCAGTAGTTGTGG           342        54.3
                13R      TATACGAACTGGAAAGATGC
RB exon 14      14F      GTGATTTTCTAAAATAGCAGG          212        58.9
                14R      TGCCTTGACCTCCTGATCTG
RB exon 15+16   15/16F   CAATGCTGACACAAATAAGG           366        55
                15/16R   AGCATTCCTTCTCCTTAACC
RB exon 17      17F      AAAAATACCTAGCTCAAGGG           339        56
                17R      TGTTAAGAAACACCTCTCAC
RB exon 18      18F      TGTACCTGGGAAAATTATGC           340        56.4
                18R      CTTTATTTGGGTCATGTACC
RB exon 19      19F      ATAATCTGTGATTCTTAGCC           273        56
                19R      AAGAAACATGATTTGAACCC
RB exon 20      20F      AAAGAGTGGTAGAAAAGAGG           335        56.4
                20R      CAGTTAACAAGTAAGTAGGG
RB exon 21      21F      AAACCTTTCTTTTTTGAGGC           328        54
                21R      TACATAATAAGGTCAGACAG
RB exon 22      22F      TAATATGTGCTTCTTACCAGTC         313        56
                22R      TTTAATGTTTTGGTGGACCC
RB exon 23      23F      ATCTAATGTAATGGGTCCAC           287        54.2
                23R      CTTGGATCAAAATAATCCCC
RB exon 24      24F      GAATATAGTTTGTCAGTGGTTC         273        52 53
                24R      GTGTTTGAATAACTGCATTTGG
RB exon 25      25F      GGTTGCTAACTATGAAACAC           297      54.2 55
                25R      AGAAATTGGTATAAGCCAGG
RB exon 26      26F      AGTAAGTCATCGAAAGCATC           209        52.8
                26R      AACGAAAAGACTTCTTGCAG
RB exon 27      27F      CGCCATCAGTTTGACATGAG           237        54.2

Abouzeid, Mol Vis 2007; 13:1740-1745 <http://www.molvis.org/molvis/v13/a194/>
©2007 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535