Table 2 of
Baharvand, Mol Vis 2007;
13:1711-1721.
Table 2. Highly abundant and reproducible protein spots identified by MALDI TOF-TOF MS analysis and Mascot search. All the identified proteins relate to Homo sapiens
The "Spot number" column indicates the number corresponding to the 2D gel in Figure 2. The "Experimental" column denotes the experimental masses measured by MS. The "Theoretical" column indicates the theoretical masses. The "% covered" indicates the percent of sequence which has been covered by MS analysis. "PMF" is the number of matched peptide resulting from peptide mass fingerprinting and MS/MS.
Mascot Spot Experimental Theoretical Gene score/ PMF/ number (Mr/pI) (Mr/pI) Protein name GenBank Swiss-Prot Symbol % covered MS-MS ------ ------------ ----------- ---------------------------- ----------- ---------- ------ --------- ----- 1 6.7/30 5.7/ 2 Collagen VI, alpha-1 gi|30032 P12109 COL6A1 169/46 10/ 6 5 6.3/21 5.7/ 29 Collagen VI, alpha-1 gi|30032 P12109 COL6A1 121/34 8/ 5 13 5.9/35 5.5/ 34 Osteoglycin gi|7661704 P20774 OGN 186/37 11/ 6 15 5.6/32 5.1/ 78 Transglutaminase 2 isoform a gi|39777597 P21980 TGM2 89/12 8/ 3 24 5.2/35 5.3/ 34 Osteoglycin gi|33150528 Q7Z532 OGN 275/28 9/ 6 26 5.4/35 5.5/ 34 Osteoglycin gi|7661704 P20774 OGN 236/40 12/ 6 27 5.6/35 5.3/ 34 Osteoglycin gi|33150528 Q7Z532 OGN 170/34 10/ 3 35 5.4/50 6.2/ 39 Lumican precursor gi|4505047 P51884 LUM 128/26 11/ 3 39 5.0/51 6.2/ 39 Lumican precursor gi|4505047 P51884 LUM 171/35 12/ 4 43 4.8/52 6.2/ 39 Lumican precursor gi|4505047 P51884 LUM 139/30 10/ 4 56 5.3/78 5.1/ 72 Heat shock 70 kDa protein 5 gi|16507237 P11021 HSPA5 1030/45 25/10 57 5.3/78 5.1/ 72 Heat shock 70 kDa protein 5 gi|16507237 P11021 HSPA5 689/23 11/ 8 93 5.6/50 6.2/ 39 Lumican precursor gi|4505047 P51884 LUM 93/28 9/ 4 106 5.6/43 5.4/205 Periplakin gi|14195005 O60437 PPL 148/13 22/ 4 107 5.6/43 5.4/ 42 beta-actin variant gi|62897625 Q53G99 ACTB 157/44 17/ 5 109 5.5/43 5.4/ 42 beta-actin variant gi|62897625 Q53G99 ACTB 175/42 17/ 5 111 5.6/50 6.2/ 39 Lumican precursor gi|4505047 P51884 LUM 90/25 9/ 3 217 5.5/40 5.4/109 Nidogen 2 (osteonidogen) gi|23273447 Q8IV28 NID2 350/12 9/ 5 355 6.5/30 5.7/ 29 Collagen VI, alpha-1 gi|30032 P12109 COL6A1 638/39 10/ 6 358 5.2/93 5.3/110 Collagen VI, alpha-1 gi|87196339 P12109 COL6A1 533/10 10/ 7 359 5.3/93 5.5/ 48 Collagen VI, alpha-1 gi|30030 P12109 COL6A1 557/37 14/ 7 370 5.4/93 5.3/110 Collagen VI, alpha-1 gi|87196339 P12109 COL6A1 714/20 16/10 |