Table 1 of
Baharvand, Mol Vis 2007;
13:1711-1721.Table 1 of
Baharvand, Mol Vis 2007;
13:1711-1721.
Table 1. Primers and conditions
Primer sequences and reaction conditions of reverse transcription-polymerase chain reaction of epithelial outgrowth of limbal explants.
Annealing Genbank
temperature Length accession
Genes Primer sequences (5'-3') (°C) (bp) number
----- ------------------------------------ ----------- ------ ---------
ABCG2 F: 5'-CTCTTCTTCCTGACGACCAACC-3' 62 312 NM_004827
R: 5'-CACACTCTGACCTGCTGCTATG-3'
KRT19 F: 5'-TGAGGTCATGGCCGAGCAGAAC-3' 69 331 NM_002276
R: 5'-CATGAGCCGCTGGTACTCCTGA-3'
KRT12 F: 5'-TGCGAGCTCTAGAAGAGGCTA-3' 62 378 NM_000223
R: 5'-CCTCGTGGTTCTTCTTCATGTA-3'
p63 F: 5'-GAGGTTGGGCTGTTCATCAT-3' 60 299 NM_003722
R: 5'-GTGGGAAAGAGATGGTCTGG-3'
Pax6 F: 5'-TCCATCAGTTCCAACGGAGAAG-3' 60 337 NM_001604
R: 5'-GTGGAATTGGTTGGTAGACACTG-3'
GAPDH F: 5'-CCAGGTGGTCTCCTCTGACTTCAACAG-3' 62 227 NM_002046
R: 5'-AGGGTCTCTCTCTTCCTCTTGTGCTCT-3'
ABCG2 F: 5'-CTCTTCTTCCTGACGACCAACC-3' 62 312 NM_004827
R: 5'-CACACTCTGACCTGCTGCTATG-3'
KRT19 F: 5'-TGAGGTCATGGCCGAGCAGAAC-3' 69 331 NM_002276
R: 5'-CATGAGCCGCTGGTACTCCTGA-3'
KRT12 F: 5'-TGCGAGCTCTAGAAGAGGCTA-3' 62 378 NM_000223
R: 5'-CCTCGTGGTTCTTCTTCATGTA-3'
p63 F: 5'-GAGGTTGGGCTGTTCATCAT-3' 60 299 NM_003722
R: 5'-GTGGGAAAGAGATGGTCTGG-3'
Pax6 F: 5'-TCCATCAGTTCCAACGGAGAAG-3' 60 337 NM_001604
R: 5'-GTGGAATTGGTTGGTAGACACTG-3'
GAPDH F: 5'-CCAGGTGGTCTCCTCTGACTTCAACAG-3' 62 227 NM_002046
R: 5'-AGGGTCTCTCTCTTCCTCTTGTGCTCT-3'
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