Table 4 of
Popp, Mol Vis 2007;
13:164-173.Table 4 of
Popp, Mol Vis 2007;
13:164-173.
Table 4. Real-time PCR results
Change in gene expression in tissue surrounding the surgical site compared to similar tissue from the non-surgically treated control eye based on real-time PCR (real-time) and microarrays (Array). Fold change values are arithmetic and not logarithmic. Also listed are the corresponding processed red and green signal values from the microarrays. The asterisk denotes signal values that are indistinguishable from background values and the double asterisk indicates signal values that are inordinately high.
Transforming growth factor beta 2
-------------------------------------------------------
Sample Green Signal Red Signal Array Real-Time
------ ------------ ---------- ------ ---------
1A 73.9** 45.3 -1.38 0.77
2A 21.2 9* -2.35 0.26
4A 850.6** 83.2 -10.20 0.04
7A 263.9** 43.7 -6.06 0.15
Mean -5.00 0.31
Matrix metalloproteinase 9
-------------------------------------------------------
Sample Green Signal Red Signal Array Real-Time
------ ------------ ---------- ----- ---------
1A 29.7 106.8 3.60 727.2
2A 18.4 158.9 8.64 1928.9
4A 26.2 69.8 2.67 574.4
7A 30.9 110.8 3.59 1020.4
Mean 4.62 1062.7
Interleukin-1 beta
-------------------------------------------------------
Sample Green Signal Red Signal Array Real-Time
------ ------------ ---------- ----- ---------
1A 14 254.8 18.25 86.03
2A 4.3* 50.4 11.69 258.92
4A 2* 11.1* 5.43 67.75
7A 17.2 306.1 17.82 146.83
Mean 13.3 139.9
Fibronectin
-------------------------------------------------------
Sample Green Signal Red Signal Array Real-Time
------ ------------ ---------- ----- ---------
1A 9.9* 24.8 2.50 5.68
2A 2.9* 6.1* 2.08 4.65
4A 4.3* 10.2* 2.36 5.36
7A 15.3 29.7 1.94 6.42
Mean 2.22 5.53
Transforming growth factor beta 1
-------------------------------------------------------
Sample Green Signal Red Signal Array Real-Time
------ ------------ ---------- ----- ---------
1A 8.8 11.5 1.30 2.43
2A 10.3 18.2 1.77 1.64
4A 14.9 12.8 -1.16 1.46
7A 12 11.3 -1.06 6.91
Mean 0.21 3.11
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