Table 6. Majority of spots on a 2D-gel contained a mixture of crystallins in the WI-UI proteins of a cataractous lens

Amino acid sequences of tryptic peptides recovered from protein spots present in two-dimensional gel electrophoretic profiles of water insoluble-urea insoluble-protein fraction from a 61-year-old human cataractous lens. The spot numbers in the WI-UI protein fractions of a cataractous lens are identified in Figure 2B. The crystallins present in each spot were identified based on the amino acid sequences of tryptic fragments.