Table 2 of
Harrington, Mol Vis 2007;
13:1680-1694.Table 2 of
Harrington, Mol Vis 2007;
13:1680-1694.
Table 2. Identification of crystallins present in protein spots of a 2D-gel of the WI-US protein fraction of a normal lens by the ES-MS/MS method
Amino acid sequences of tryptic peptides recovered from protein spots present in two-dimensional gel electrophoretic profiles of water insoluble-urea soluble protein fraction isolated from the normal lens of a 68-year-old donor. The spot numbers in the table correspond to those shown in Figure 1A in the WI-US fraction of a normal lens. The amino acid sequences reported are those of tryptic fragments from individual spots.
Spot
number α-Crystallin α- and α-Crystallin Filensin Vimentin
------ ----------------------------------------------------- -------------------------------------------------- --------------------------------------- -----------------------------------------
1 αB: Ac-MDIAIHHPWIR (#1-11, 2 Oxid. M) γD: FNEIHSLNVLEGSWVLYELSNYR (#118-140)
αB: Ac-MDIAIHHPWIR (#1-11, Oxid. M)
αB: DIAIHHPWIR (#2-11)
αB: APSWFDTGLSEMR (#57-69, Oxid. M)
αB: APSWFDTGLSEMR (#57-69, Oxid. M, 2 OH groups on W
αB: APSWFDTGLSEMRLE (#57-71, Oxid. M; PO3H on S66
αB: APSWFDTGLSEMRLEK (#57-72, Oxid. M)
αB: APSWFDTGLSEMRLEKDR (#57-74, Oxid. M)
αB: DRFSVNLDVK (#73-82)
αB: FSVNLDVK (#75-82)
αB: KYRIPADVDPLTITSSLSSDGVLTVNGPR (#121-149)
αB: KYRIPADVDPLTITSSLSSDGVLTVNGPRKQVSGPER (#121-157
αB: YRIPADVDPLTITSSLSSDGVLTVNGPRKQVSGPER (#122-157)
αB: IPADVDPLTITSSLSSDGVLTVNGPRK (#124-150)
αB: IPADVDPLTITSSLSSDGVLTVNGPR (#124-149)
αB: TIPITREEKPAVTAAPK (#158-174)
αB: PITREEKPAVTAAPK (#160-174)
αB: EEKPAVTAAPK (#164-174)
2 αB: Ac-MDIAIHHPWIR (#1-11, Oxid. M) βB1: LVVFELENFQGR (#60-71)
αB: DIAIHHPWIR (#2-11)
αB: AIHHPWIR (#4-11)
αB: RPFFPFHSPSR (#12-22)
αB: PFHSPSR (#16-22)
αB: APSWFDTGLSEMR (#57-69)
αB: APSWFDTGLSEMR (#57-69, 2 Oxid. M)
αB: APSWFDTGLSEMR (#57-69, Oxid. M; OH on W)
αB: APSWFDTGLSEMR (#57-69, Oxid. M)
αB: APSWFDTGLSEMRLE (#57-71, Oxid. M; PO3H on S66)
αB: APSWFDTGLSEMRLEK (#57-72, Oxid. M)
αB: APSWFDTGLSEMRLEKDR (#57-74, Oxid. M)
αB: SEMRLEKDR (#66-74, Oxid. M)
αB: LEKDRFSVNLDVK (#70-82)
αB: FSVNLDVK (#75-82)
αB: VLGDVIEVHGKHEERQDEHGFISR (#93-116)
αB: GDVIEVHGKHEERQDEHGFISR (#95-116)
αB: IPADVDPLTITSSLSSDGVLTVNGPR (#124-149)
αB: TIPITREEKPAVTAAPK (#158-174)
αB: PITREEKPAVTAAPK (#160-174)
3 αB: Ac-MDIAIHHPWIR (#1-11, Oxid. M; OH on W) βA3: ITIYDQENFQGK (#33-44)
αB: Ac-MDIAIHHPWIR (#1-11, Oxid. M) βA3: MTIFEKENFIGR (#126-137, Oxid. M)
αB: DIAIHHPWIR (#2-11, OH on W)
αB: DIAIHHPWIR (#2-11) βB2: IILYENPNFTGK (#108-119)
αB: AIHHPWIR (#4-11)
αB: RPFFPFHSPSR (#12-22, PO3H on S19) βS: VLEGVWIFYELPNYR (#131-145)
αB: RPFFPFHSPSR (#12-22)
αB: PFHSPSR (#16-22)
αB: LFDQFFGEHLLESDLFPTSTSLSPFYLRPPSFLR (#23-56)
αB: APSWFDTGLSEMR (#57-69, Oxid. M; PO3H on S59)
αB: APSWFDTGLSEMR (#57-69, Oxid. M)
αB: APSWFDTGLSEMR (#57-69, Oxid. M)
αB: APSWFDTGLSEMRLE (#57-71, Oxid. M; PO3H on S66)
αB: APSWFDTGLSEMRLEK (#57-72, Oxid. M)
αB: APSWFDTGLSEMRLEK (#57-72, Oxid. M; PO3H on S59)
αB: APSWFDTGLSEMRLEKDR (#57-74, Oxid. M; PO3H on S59)
αB: APSWFDTGLSEMRLEKDR (#57-74, Oxid. M)
αB: SEMRLEKDR (#66-74, Oxid. M)
αB: DRFSVNLDVK (#73-82)
αB: LEKDRFSVNLDVK (#70-82)
αB: LEKDRFSVNLDVK (#70-82, PO3H on S76)
αB: HFSPEELK (#83-90)
αB: VLGDVIEVHGKHEERQDEHGFISR (#93-116)
αB: GDVIEVHGKHEERQDEHGFISR (#95-116)
αB: KYRIPADVDPLTITSSLSSDGVLTVNGPR (#121-149)
αB: YRIPADVDPLTITSSLSSDGVLTVNGPR (#122-149)
αB: IPADVDPLTITSSLSSDGVLTVNGPR (#124-149)
αB: IPADVDPLTITSSLSSDGVLTVNGPR (#124-149)
αB: IPADVDPLTITSSLSSDGVLTVNGPR (#124-149)
+ Na
αB: PLTITSSLSSDGVLTVNGPRKQVSGPER (#130-157)
αB: SSLSSDGVLTVNGPRKQVSGPER (#135-157)
αB: TIPITREEKPAVTAAPK (#158-174)
αB: TIPITREEKPAVTAAPK (#158-174)
αB: PITREEKPAVTAAPK (#160-174)
4 αB: Ac-MDIAIHHPWIR (#1-11, Oxid. M) βA3: ITIYDQENFQGK (#33-44)
αB: DIAIHHPWIR (#2-11) βA3: MEFTSSCPNVSERSFDNVR (#46-64, Oxid. M)
αB: APSWFDTGLSEMR (#57-69, Oxid. M; PO3H on S59)
βA3: GEYPRWDAWSGSNAYHIER (#91-109)
αB: APSWFDTGLSEMR (#57-69, Oxid. M)
αB: APSWFDTGLSEMRLEK (#57-72, Oxid. M) βA3: WDAWSGSNAYHIER (#96-109)
αB: YRIPADVDPLTITSSLSSDGVLTVNGPR (#122-149) βA3: IQSGAWVCYQYPGYR (#163-177)
βA4: LTIFEQENFLGK (#107-118)
βB1: LVVFELENFQGR (#60-71)
βB1: ISLFEGANFK (#150-159)
βB1: APSLWVYGFSDR (#170-181)
βB1: VSSGTWVGYQYPGYR (#187-201)
βB1: GYQYLLEPGDFR (#202-213)
βB1: HWNEWGAFQPQMQSLR (#214-229, Oxid. M)
5 αB: Ac-MDIAIHHPWIR (#1-11, Oxid. M) βA3: ITIYDQENFQGK (#33-44)
αB: APSWFDTGLSEMR (#57-69, Oxid. M) βA3: MEFTSSCPNVSERSFDNVR (#46-64, Oxid. M)
αB: APSWFDTGLSEMR (#57-69)
αB: APSWFDTGLSEMR (#57-69, Oxid. M; PO3H on S59) βA3: PNVSERSFDNVR (#53-64)
βA3: GEYPRWDAWSGSNAYHIER (#91-109)
αB: APSWFDTGLSEMR (#57-69, PO3H on S59)
αB: HFSPEELK (#83-90) βA3: WDAWSGSNAYHIER (#96-109)
βA3: SGSNAYHIER (#100-109)
βA3: MTIFEKENFIGR (#126-137, Oxid. M)
βA3: MTIFEKENFIGR (#126-137)
βA3: FEKENFIGR (#129-137)
βA3: QWEISDDYPSLQAMGWFNNEVGSMK (#138-162, Oxid. M)
βA3: IQSGAWVCYQYPGYR (#163-177)
βA3: EWGSHAQTSQIQSIR (#197-211)
βA4: LTIFEQENFLGK (#106-117)
βB1: LVVFELENFQGR (#60-71)
βB1: GYQYLLEPGDFR (#202-213)
6 βA3: ITIYDQENFQGK (#33-44)
βA3: TIYDQENFQGKR (#34-45)
βA3: IYDQENFQGKR (#35-45)
βA3: WDAWSGSNAYHIER (#96-109)
βA3: AWSGSNAYHIER (#98-109)
βA3: MTIFEKENFIGR (#126-137)
βA3: MTIFEKENFIGR (#126-137, Oxid. M)
βA3: IQSGAWVCYQYPGYR (#163-177)
βA4: VLSGAWVGFEHAGF (#48-61)
βA4: LTIFEQENFLGK (#106-117)
7 αA: TVLDSGISEVR (#55-65) βA3: ITIYDQENFQGK (#33-44) Filensin: LGELAGPEDALAR (#78-90)
αB: HFSPEELK (#83-90) βA3: ITIYDQENFQGKR (#33-45)
βA3: TIYDQENFQGKR (#34-45)
βA3: IYDQENFQGKR (#35-45)
βA3: RMEFTSSCPNVSERSFDNVR (#45-64, Oxid. M)
βA3: MEFTSSCPNVSERSFDNVR (#46-64, Oxid. M)
βA3: MEFTSSCPNVSERSFDNVR (#46-64)
βA3: GEYPRWDAWSGSNAYHIER (#91-109)
βA3: WDAWSGSNAYHIER (#96-109)
βA3: MTIFEKENFIGR (#126-137, Oxid. M)
βA3: EWGSHAQTSQIQSIR (#197-211)
βA4: MVVWDEDGFQGR (#13-24)
βA4: VLSGAWVGFEHAGFQGQQYILER (#48-70)
βA4: LTIFEQENFLGK (#108-118)
βA4: IFEQENFLGKK (#109-119)
βB1: LVVFELENFQGR (#60-71)
βB1: ISLFEGANFK (#150-159)
βB1: VSSGTWVGYQYPGYR (#187-201)
βB1: GYQYLLEPGDFR (#202-213)
8 βB1: LVVFELENFQGR (#60-71)
βB1: ISLFEGANFK (#150-159)
βB1: GYQYLLEPGDFR (#202-213)
βB1: HWNEWGAFQPQMQSLR (#214-229, Oxid. M)
βB1: DKQWHLEGSFPVLATEPPK (#233-251)
βB2:ASDHQTQAGKPQSLNPK (#1-17)
βB2: AGSVLVQAGPWVGYEQANCKGEQFVFEKGEYPR (#48-80)
βB2: WDSWTSSR (#81-88)
βB2: TDSLSSLRPIKVDSQEHK (#90-107)
βB2: SSLRPIKVDSQEHK (#94-107)
βB2: IILYENPNFTGK (#108-119)
βB2: LYENPNFTGKK (#110-120)
βB2: KMEIIDDDVPSFHAHGYQEK (#120-139)
βB2: MEIIDDDVPSFHAHGYQEK (#121-139)
βB2: MEIIDDDVPSFHAHGYQEK (#121-139, Ox. M)
βB2: PSFHAHGYQEK (#129-139)
βB2: VQSGTWVGYQYPGYR (#145-159)
βB2: VQSGTWVGYQYPGYR (#145-159, OH on W)
βB2: GLQYLLEKGDYKDSSDFGAPHPQVQSVR (#160-187)
βB2: QYLLEKGDYKDSSDFGAPHPQVQSVR (#162-187)
βB2: GAPHPQVQSVR (#177-187)
9 βA3: ITIYDQENFQGK (#33-44)
βB1: LVVFELENFQGR (#60-71)
βB1: AEFSGECSNLADRGFDR (#73-89)
βB1: GEMFILEKGEYPR (#110-122, Ox. M)
βB1: ISLFEGANFK (#150-159)
βB1: VSSGTWVGYQYPGYR (#187-201)
βB1: GYQYLLEPGDFR (#202-213)
βB1: HWNEWGAFQPQMQSLR (#214-229, Oxid. M)
βB1: DKQWHLEGSFPVLATEPPK (#233-251)
βB2: WDSWTSSR (#81-88)
βB2: TDSLSSLRPIK (#90-100)
βB2: TDSLSSLRPIKVDSQEHK (#90-107)
βB2: SSLRPIKVDSQEHK (#94-107)
βB2: IILYENPNFTGK (#108-119)
βB2: LYENPNFTGKK (#110-120)
βB2: KMEIIDDDVPSFHAHGYQEK (#120-139, Ox. M)
βB2: PSFHAHGYQEK (#129-139)
βB2: VQSGTWVGYQYPGYR (#145-159)
βB2: GLQYLLEK (#160-167)
βB2: FGAPHPQVQSVR (#176-187)
βB2: GAPHPQVQSVR (#177-187)
10 βB1: LVVFELENFQGR (#61-72)
βB1: ISLFEGANFK (#151-160)
βB1: VSSGTWVGYQYPGYR (#188-202)
βB1: GYQYLLEPGDFR (#203-214)
βB1: HWNEWGAFQPQMQSLR (#215-230, Oxid. M)
βB2: IILYENPNFTGK (#108-119)
11 βB1: LVVFELENFQGR (#60-71) Filensin: LGELAGPEDALAR (#78-90)
βB1: VFELENFQGRR (#62-72)
βB1: ISLFEGANFK (#150-159)
βB1: VSSGTWVGYQYPGYR (#187-201)
βB1: GYQYLLEPGDFR (#202-213)
βB1: HWNEWGAFQPQMQSLR (#214-229, Oxid. M)
βB1: QWHLEGSFPVLATEPPK (#235-251)
βB2: IILYENPNFTGK (#108-119)
12 αB: Ac-MDIAIHHPWIR (#1-11, Oxid. M) βA4: MVVWDEDGFQGR (#13-24, Ox. M) Filensin: LGELAGPEDALAR (#78-90)
αB: APSWFDTGLSEMR (#57-69, Oxid. M) Filensin: LNKEADEALLHNLR (#144-157)
αB: HFSPEELK (#83-90) βB1: AAELPPGNYR (#50-59) Filensin: LQLEAQFLQDDISAAKDR (#158-175)
βB1: LVVFELENFQGR (#60-71)
βB1: LVVFELENFQGRR (#60-72)
βB1: AEFSGECSNLADR (#73-85)
βB1: LMSFRPIK (#135-142, Ox. M)
βB1: ISLFEGANFK (#150-159)
βB1: VSSGTWVGYQYPGYR (#187-201)
βB1: GYQYLLEPGDFR (#202-213)
βB1: HWNEWGAFQPQMQSLR (#214-229, Oxid. M)
βB1: DKQWHLEGSFPVLATEPPK (#233-251)
13 Filensin: LGELAGPEDALAR (#78-90)
Filensin: VRDLEAER (#99-106)
14 αA: TVLDSGISEVR (#55-65) βB1: LVVFELENFQGR (#60-71) Filensin: LGELAGPEDALAR (#78-90) Vimentin: SLYASSPGGVYATR (#50-63)
βB1: ISLFEGANFK (#150-159)
Vimentin: ILLAELEQLKGQGK (#129-142)
Vimentin: MALDIEIATYR (#390-400, Oxid. M)
Vimentin: ISLPLPNFSSLNLR (#410-423)
15 Filensin: LGELAGPEDALAR (#78-90)
Filensin: VRDLEAER (#99-106)
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