Table 1 of
Zhang, Mol Vis 2007;
13:1674-1679.Table 1 of
Zhang, Mol Vis 2007;
13:1674-1679.
Table 1. Primers for amplification of the FRMD7 gene
The primers were selected such that the amplicons contain coding sequence (exons) and the flanking splice junctions. In case of exons 12, nested primers (including 12a, 12b, 12c, 12d, 12e, and 12f) were used for sequencing the amplicons for better fidelity of the data generated.
Product
length
Exon Sense primers (5'-3') Antisense primers (5'-3') (bp)
---- ------------------------- ------------------------- -------
1 CCTTGGGTGTGCATTACTTC TTTGCTATTGTTGTCCCTTGAG 459
2 AAACAACACAGAGACAGATAAGTGG CAATCAGGGAATTGAACCCTAC 385
3 AGGCAGTGGAGCAGTGATTC GCAGCATGATTTCTTTCATCTC 499
4 CTCGAAGGCAGAGAGGGTAG CCCTTTGGATGATGAACACC 519
5 GGCACCATTCCTTTCTTGAAT CAGGCCATGCTGTTTCTCTC 350
6 TTTGGACTGCATTGGCTACA AGGATCTCAGCGTTTCATGG 353
7 TCATGCACTTTCATCAGAAGC TGATTGACCATTTCCCTTTC 497
8 TGTGCAAGAGATGGGTCAAG CTCTGGTTGATTTCTTCAAAGG 368
9 GCTCTGTTTGTGAGCAGTGG AGGGTGCAATCTTTGATGTG 495
10 AGGTTGTTCTCTGCCTGGTC GCACTGTCGTTCATGGTACTG 398
11 TGTTTCTCTTGCTCGTGTTGA TTTTTACACACTGGGATTCTGG 282
12a CCCTAGAATAGAACATGGATCTTG TGGGATCAGGGTTAGGATTG 388
12b CCTTCTTTCACCAATGTGTCC AATACCAACCTGCTGACCTG 452
12c CTTTAACACTGAGCCCAATC TGACTGAGAGCAGGACAAGG 588
12d ACGGATGTGCCCTATATTCC GCAACTCCTGCTCTGCAAAC 472
12e AGCCCAAGGAATATCAGAATG GCAGTTGGTGTGTTGAAATAAGC 500
12f AGCCCAAGGAATATCAGAATG GCAGTTGGTGTGTTGAAATAAGC 500
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