Figure 4 of Samadi, Mol Vis 2007; 13:1618-1626.

Figure 4. Ceramide induces apoptosis in lens epithelial cells

BLEC (A) and HLEC (B) were treated in the absence or presence of C2-ceramide (5-20 μM) for indicated times. Levels of apoptosis were quantitatively determined by DAPI nuclear staining as described in Methods. Values are expressed as percentage of apoptotic cells. C illustrates the effect of ceramide-induced apoptosis determined by Cell Death DetectionPLUS ELISA assay as described in Methods. BLEC were treated with C2-ceramide (0, 5, and 10 μM) for 24 h. In each case, the data represent mean±SEM from three separate experiments. The single asterisk denotes a p<0.05 and the double asterisk indicates a p<0.001.

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