Table 3. Primers used for sequencing the MYOC gene

Primers used in amplification of MYOC exons were also used in sequencing reactions. Primers located in introns were placed far enough away from the exon boundaries to allow visualization of the sequence of the splice sites. Primer pairs 1A, 2, and 3A were used for PCR amplification and sequencing of exons 1, 2, and 3, respectively. Primers 1B and 3B are internal primers that were used for sequencing purposes only.

        Forward primer sequence     Reverse primer sequence
Exon            (5'-3')                     (5'-3')
----   -------------------------   -------------------------
 1A    GGCTGGCTCCCCAGTATATA        CTGCTGAACTCAGAGTCCCC
 1B    AGGCCAATGTCAAGTCATCCAT      CTCCAGAACTGACTTGTCTC
  2    ACATAGTCAATCCTTGGGCC        TAAAGACCACGTGGCACA
 3A    CTGGCTCTGCCAAGCTTCCGCATGA   GGCTGGCTCTCCCTTCAGCCTGCT
 3B    GAGCTGAATACCGAGACAGTGAA     GAGGCCTGCTTCATCCACAGCCAAC