Table 2 of
Ayala-Lugo, Mol Vis 2007;
13:151-163.Table 2 of
Ayala-Lugo, Mol Vis 2007;
13:151-163.
Table 2. Primers used for sequencing the OPTN gene
Primers used in amplification of OPTN exons were also used in sequencing reactions. Primers located in introns were placed far enough away from the exon boundaries to allow visualization of the sequence of the splice sites. Exons 4 through 16 are the exons that contain coding sequence.
Forward primer sequence Reverse primer sequence
Exon (5'-3') (5'-3')
---- ----------------------- -----------------------
4 TGGAGAGAAAGTGGGCAACT CACCAGCTACCACCTATGGA
5 GGCATCTTTCAATTCAGAGCC GACACGTAAGATTCCACTGC
6 TCCCAGAGCTCTGCGATTAA GCTACACTGGAATTTCCTCA
7 TCTGAGCCACCCCGTTTAAA GACCTCCGGTGACAAG
8 GGAGAATGTTCTGGAAAGCAG GGGTGAACTGTATGGTATCT
9 CCCCTGATCCTTTATCCCAA AATTCAGTGGCTGGACTAC
10 TGGTTCAGCCTGTTTTCTCC CCCCCCATCTTACAAGTATTTC
11 TGGCCAGGTCTAGTGAAGAA TTTATCCCCCTCTCTGAGAG
12 GAAATGCTAGTAGGTCGTGG CCCTGACCATAGGACATTCA
13 CCGGCCAGAGCTGATAAT AGATCCACTGAGCACTTTCC
14 CTAGCAGGATTGTGCATCGT GTGGCGCGAACACAGCTATT
15 TTTCCCCTACTTCTGTGGAC GAGACTGACGGGTGCTATAT
16 TCATGTCCCACTACGTGTTG TGTGCCCGGCCTGTTTTCTT
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