Table 3 of
Soest, Mol Vis 2007;
13:1608-1617.Table 3 of
Soest, Mol Vis 2007;
13:1608-1617.
Table 3. Results of retinal pigment epithelium microarray and confirmatory reverse transcriptase polymerase chain reaction experiments
The following abbreviations were used: macular area (M), paramacular area (P1), mid-postequatorial periphery (P2), fold change in gene expression observed between M and P1 (M/P1), and fold change in gene expression observed between M and P2 (M/P2). Genes with a fold change of 3.4 or higher in the microarray experiments are shown, sorted on M/P2. In the reverse transcriptase polymerase chain reaction results, numbers in between parentheses denote fold changes that were not statistically significant. (A p>0.01 was considered significant for microarray experiments and p>0.05 was considered relevant for real-time PCR experiments). Positive numbers relate to higher RPE expression in the macula compared to the periphery. Negative numbers relate to lower gene expression in the macular RPE. Some experiments were not done (n.d.) for several reasons, such as low gene expression, suitable primers could not be developed, low priority target (choice), etc.
Genbank acc Gene description Array M/P1 RT-PCR M/P1 Array M/P2 RT-PCR M/P2 ----------- ----------------------------------------------------------- ---------- ----------- ---------- ----------- NM_020346 Solute carrier family 17 (sodium-dependent), member 6 3.2 6.3 110 14.9 NM_021197 WAP four-disulfide core domain 1 7.6 6.8 25.6 37.1 NM_021076 Neurofilament, heavy polypeptide 200kDa 3.2 n.d. 9.4 n.d. NM_005382 Neurofilament 3 (150kDa medium) 4.2 6.7 9.3 17.1 NM_004887 Chemokine (C-X-C motif) ligand 14 2.3 9 7.4 8.6 NM_000513 Opsin 1 (cone pigments), medium-wave-sensitive 4.1 n.d. 6 n.d. NM_002522 Neuronal pentraxin I 3 -2.3 -4.8 -2.1 NM_020061 Opsin 1 (cone pigments), long-wave-sensitive 3.1 n.d. -4.6 n.d. AF268897 Voltage gated potassium channel Kv3.2a 3.8 -2.4 -4.2 -2.8 NM_020980 Aquaporin 9 2.6 -16.5 4.2 -19.3 NM_015900 Phospholipase A1 member A 2 -3.9 4.1 -5.7 NM_024563 Hypothetical protein FLJ14054 (-1) n.d. 3.8 n.d. NM_002632 Placental growth factor, VEGF-related protein -1.4 n.d. 3.7 n.d. NM_002048 Growth arrest-specific 1 -2 (-1.2) 3.7 4.4 NM_007029 Stathmin-like 2 2.9 n.d. 3.7 n.d. NM_018098 Epithelial cell transforming sequence 2 oncogene 3.2 n.d. -3.7 n.d. NM_001708 Opsin 1 (cone pigments), short-wave-sensitive 4.3 n.d. 3.6 n.d. NM_006688 Complement component 1, q subcomponent-like 1 3 -1.2 3.6 -1.4 NM_031498 Guanine nucl.bind. protein, gamma transd. act. polyp 2 2.9 n.d. 3.6 n.d. NM_006205 Phosphodiesterase 6H, cGMP-specific, cone, gamma 2.2 n.d. -3.5 n.d. BG396167 Guanine nucl. bind. protein, gamma transd. act. polyp 2 -1.6 n.d. 3.4 n.d. NM_020465 NDRG family member 4 -2.5 n.d. 3.4 n.d. NM_002075 Triosephosphate isomerase 1 2.2 4.1 3.4 6.5 NM_004398 DEAD (Asp-Glu-Ala-Asp) box polypeptide 10 3.2 n.d. 3.4 n.d. NM_003713 Phosphatidic acid phosphatase type 2B (-1.3) n.d. -3.4 n.d. NM_001145 Ribonuclease, RNase A family, 4 (-2.7) n.d. -3.4 n.d. AJ420521 SPARC related modular calcium binding 2 (-1.5) n.d. -3.4 n.d. NM_012385 p8 protein (candidate of metastasis 1) (-3.2) n.d. -3.5 n.d. NM_006528 Tissue factor pathway inhibitor 2 -2.7 (-1.7) -3.5 -6.8 NM_004126 Guanine nucleotide binding protein, gamma 11 (-2.1) n.d. (-3.7) n.d. NM_004484 Glypican 3 (-1.8) n.d. -3.7 n.d. NM_016192 Transmembr. Prot., EGF-like, two follistatin-like domains 2 (-1.2) n.d. -3.7 n.d. NM_000627 Latent transforming growth factor beta binding protein 1 (-1.9) (-2.3) -3.7 -4.5 NM_000860 Hydroxyprostaglandin dehydrogenase 15-(NAD) (-1.1) n.d. -3.7 n.d. NM_006072 Chemokine (C-C motif) ligand 26 (-3.1) (-9.5) -3.8 -17.1 AB033025 KIAA1199 -2.1 n.d. -3.9 n.d. NM_015429 ABI gene family, member 3 (NESH) binding protein (-1.2) n.d. -3.9 n.d. NM_003239 Transforming growth factor, beta 3 (-2.2) -1.2 -4 (-1.1) NM_006206 Platelet-derived growth factor receptor, a-polypeptide (-1.5) (-2.8) -4.1 -5.9 NM_018022 Transmembrane protein 51 -2 (-1.3) -4.1 (-1.9) NM_000325 Paired-like homeodomain transcription factor 2 (-1.5) n.d. -4.1 n.d. NM_012105 Beta-site APP-cleaving enzyme 2 (-3.1) n.d. -4.1 n.d. NM_016651 Dapper, antagonist of beta-catenin, homolog 1 (X.laevis) (-1.8) (-1.2) -4.2 (-2.0) NM_000428 Latent transforming growth factor beta binding protein 2 (-2.7) (-1.0) -4.3 (-1.3) NM_001797 Cadherin 11, type 2, OB-cadherin (osteoblast) (-1.5) (-2.2) -4.4 (-1.7) AK055903 KIT ligand (-1.1) (-2.4) -4.4 -3.8 AL136693 Cytochrome b reductase 1 -1.4 -3.1 -4.4 -4.2 NM_031950 Ksp37 protein (-3.4) -2.5 -4.5 -1.6 NM_004360 Cadherin 1, type 1, E-cadherin (epithelial) (-2) (-2.7) -4.6 -9.1 NM_001647 Apolipoprotein D (-2.7) (-2.9) -4.7 -6.8 NM_000275 Oculocutaneous albinism II (pink-eye dil.hom., mouse) (-2) (-2.0) -5 (-4.2) AL162032 G protein-coupled receptor 133 (-1.8) n.d. -5.1 n.d. NM_000582 Secreted phosphoprotein 1 (osteopontin) (-4.6) -1.6 -5.7 -1.4 NM_006404 Protein C receptor, endothelial (EPCR) (-3) -1.5 -5.8 -1.1 NM_018965 Triggering receptor expressed on myeloid cells 2 (-4.1) -4.2 -6.2 -2.4 AK025719 Insulin-like growth factor 2 (somatomedin A) (-2.2) (-1.6) -6.5 (-2.6) NM_003890 Fc fragment of IgG binding protein (-1.6) -5.8 -7.4 10.2 NM_000222 V-kit Hardy-Zuckerman 4 feline sarc.vir.oncogene homol. -2.9 (-2.4) -7.9 -5.2 L38486 Microfibrillar-associated protein 4 -2.2 (-1.1) -8.8 -5.1 NM_002153 Hydroxysteroid (17-beta) dehydrogenase 2 -24.1 (-16.7) -8.8 -16.1 NM_001362 Deiodinase, iodothyronine, type III -56.6 (-3.1) -9.9 (-1.6) NM_014057 Osteoglycin (osteoinductive factor, mimecan) (-1) (-4.5) -12.7 -16.2 NM_000261 Myocilin, trabecular meshw. Ind.glucocorticoid resp.pr. -9.4 (-12.2) -13.3 -30.5 NM_000693 Aldehyde dehydrogenase 1 family, member A3 -8.2 (-1.7) -14.4 -29.7 NM_003014 Secreted frizzled-related protein 4 (-3.4) (-4.7) -15.1 -17 NM_009590 Amine oxidase, copper containing 2 (retina-specific) -39.9 n.d. (-19.6) n.d. |