Ann
                                                                temp
Exon   Forward primer 5'- 3'     Reverse primer 5' - 3'   bp    (°C)
----   -----------------------   ----------------------   ---   ----
1      GACCAATCTGGTCTTCGTG       GTTTATTTGCTCCACACCTC     145   56
2      TAGCACCACTGAACTTTCTCT     AAGGCCCAGACCAAAGTCTC     191   58
3      CCTGCTTGGTCTCCATGAC       ACGTGAAGGGGTGTGCAAC      249   58
4      GCTATTTCCTTATTAATGAGGC    CCAACTCTCCCTGTTCTTTC     259   58
5      GACCCATTTCCCCTTCAAC       AGGCTGGGTGCTTCCCTC       230   56
6      CTTTCCTACCACAGGGCAG       AGGAATCACCTTGCAATTGG     289   58
7      TGCCTATGTGTGTATATACC      TAAGTGGGGTAAATGGTGG      220   58
8      GAGCATTGGCCTCACAGCAG      TTAACCAACATGAGAGGCC      356   56
9      AAGCAATGGGGAGTTTCTGT      GAGATGTGATACCAGGAAG      289   58
10     GACACACCAAAAGTTCTCTCT     TCCCCTCCCCTCCCCATC       222   58
11     CTAAGCAGAGCAGTGACTG       ACTTGACTTGCTAAGGGAG      314   58
12     GGTCCTCCTCACACTCTCT       ATTTCCCACTGACTTTGGAG     286   58
13     GAGGTGTGAGTGAGCTATCC      CCCATTAGCGTGTCATGG       282   58
14     CCTCTACCAGGTACAGAGC       GGGAAAGGAACCAAAGTATTC    330   58
15     AGGCTGGTGGGAGAGAGC        AGTGGACCCCCTCAGAGG       407   58
16     CTGTTGCATTGGATAAAAGGC     GATGAATGGAGAGGGCTGG      330   58
17     CTGCGGTAAGGTAGGATAGGG     CACACCGTTTACATAGAGGGC    232   58
18     CCTCTCCCCTCCTTTCCTG       GTCAGTTTCCGTAGGCTTC      279   58
19     TGGGGCCATGTAATTAGGC       TGGGAAAGAGTAGACAGCCG     322   58
20     ACTGAACCTGGTGTGGGG        TATCTCTGCCTGTGCCCAG      325   60
21     GTAAGATCAGCTGCTGGAAG      GAAGCTCTCCTGCTCCAAGC     301   58
22     CACCCTCCACAGCCCCTTAAC     TCGTTGTGGTTCCTGTACTCAG   291   58
23     TTTTTGCAACTATATAGCCAGG    AGCCTGTGTGAGTAGCCATG     384   58
24     GCATCAGGGAGAGGCTGTC       CCCAGCAATATTGGGAGATG     212   54
25     GGTAACCTCACAGTCTTCC       GGGAACGATGGCTTTTTGC      379   58
26     CAAAACAGAGCTGGGTTAG       ACTTTCGAGATGGAACTTGG     191   58
27     GCTACCAGCCTGGTATTTCATTG   GTTATAACCCATGCCTGAAG     493   56
28     CCACCAGGGGCTGATTAG        CCCAAACCCACACAGAGGAG     289   58
29     GTTGCATGATGTTGGCACG       TCTTAGGACAGGGGCGCG       185   58
30     GTCAGCAACTTTGAGGCTG       ACTCAGGAGATACCAGGGAC     314   58
31     TAAGTCCTCAAGTTCCAAGG      TCTTCTACAGGGCAGCCAG      193   58
32     GAAAGTTAACGGCACTGCT       CATGGATGTGAGGTGTGC       185   58
33     TTCATGTTTCCCTACAAAACCC    CATGAGAGTTTCTCATTCATGG   265   58
34     GCTTAACTACCATGAATGAG      ATTCCTTGCTAGATTTCAGC     286   56
35     GCAGCGTCTCCAATGTCCTC      AAGAGTGGAGAAGGTGACAAG    255   58
36     GTATCTTCTCCTCCTTGTGC      ACACACAAGCTCCACCTTG      304   58
37     TTGCAGAGCTGGCAGCAG        CCACCAGGCTTCTCTTCAG      226   58
38     GGAATGGAATGTGGAACTCC      CACATACTCTACTATCCTAC     253   58
39     TGCTGTCCTGTGAGAGCATC      TCCCAGCTTTGGACCCAG       268   58
40     CCAGGTCTGTGGGGTGAG        AGTTCTGGATGCCCTGAG       241   60
41     GGACACTGTACAGCCAGC        GACGAGTTATAACACAGGG      319   58
42     CTCCTAAACCATCCTTTGCTC     AGGCAGGCACAAGAGCTG       214   58
43     CTTACCCTGGGGCCTGAC        CTCAGAGCCACCCTACTATAG    277   58
44     GAAGCTTCTCCAGCCCTAGC      TGCACTCTCATGAAACAGGC     287   58
45     GTTTGGGGTGTTTGCTTGTC      ACCTATTTCCCCAACCCAAGAG   257   58
46     GAAGCAGTAATCAGAAGGGC      GCCTCACATTCTTCCATGCTG    256   58
47     TCACATCCCACAGGCAAGAG      AGGTGGATCCACAGAAGGC      256   58
48     GATTACCTTAGGCCCAACC       ACACTGGGTGTTCTGGACC      228   60
49     GTGTAGGGTGGTGTTTTCC       AAGCCCAGTGAACCAGCTGG     365   58