Table 1 of
Yuan, Mol Vis 2007;
13:1555-1561.
Table 1. Oligonucleotides used for PAX6 amplification
Sequence of oligonucleotides and annealing temperatures used for exon-by-exon PAX6 amplification.In the table, "F" indicates the forward strand and "R" indicates the reverse strand. Exon 13 was amplified in two overlapping fragments. In each case, primer pair (1)F and(1)R amplifies the most upstream 5' part of the target genomic DNA, and primer pair, (2)F and (2)R, amplifies the most downstream 3' part of the target genomic DNA encompassing the specific exonic sequences. Sequences are given in the 5'-3' direction.
Annealing temperature Exon Sequences(5'-3') (°C) ------- ------------------------ ----------- 3,4 F:GGACTTAGGGTTTGATGACAG 50 3,4 R:CCAGAAAGACCAGAGGCAC 5 F:GAGATTGGCACAGGTTGG 60 5 R:CATAAGTAGCATCGTTTACAGT 5a,6 F:AACGCCACTTTAAGCAAGGT 52 5a,6 R: GGAGGGCAGATGTTCTCAA 7 F:AATCCACCCACTGTCCCG 60 7 R:CCAGCCACCTTCATACCG 8 F:TCAGGTAACTAACATCGCA 53 8 R:GTTGACTGTACTTGGAAGAA 9,10,11 F: GAGGTGGGAACCAGTTTGATG 52 9,10,11 R: CAAGCCAATCTCTGTAGTGCG 12 F:GAGGCTTGATACATAGGC 52 12 R:CCATAAGACCAGGAGATT 13(1) F:GTTTCTGAGGGTGCTACT 48 13(1) R:TTGAATGGCTAACTGGG 13(2) F:CAGTTTCTGAAGGTGCTA 48 13(2) R:TCCATCCAGTCTACATTG |