Table 1 of
Yuan, Mol Vis 2007;
13:1555-1561.Table 1 of
Yuan, Mol Vis 2007;
13:1555-1561.
Table 1. Oligonucleotides used for PAX6 amplification
Sequence of oligonucleotides and annealing temperatures used for exon-by-exon PAX6 amplification.In the table, "F" indicates the forward strand and "R" indicates the reverse strand. Exon 13 was amplified in two overlapping fragments. In each case, primer pair (1)F and(1)R amplifies the most upstream 5' part of the target genomic DNA, and primer pair, (2)F and (2)R, amplifies the most downstream 3' part of the target genomic DNA encompassing the specific exonic sequences. Sequences are given in the 5'-3' direction.
Annealing
temperature
Exon Sequences(5'-3') (°C)
------- ------------------------ -----------
3,4 F:GGACTTAGGGTTTGATGACAG 50
3,4 R:CCAGAAAGACCAGAGGCAC
5 F:GAGATTGGCACAGGTTGG 60
5 R:CATAAGTAGCATCGTTTACAGT
5a,6 F:AACGCCACTTTAAGCAAGGT 52
5a,6 R: GGAGGGCAGATGTTCTCAA
7 F:AATCCACCCACTGTCCCG 60
7 R:CCAGCCACCTTCATACCG
8 F:TCAGGTAACTAACATCGCA 53
8 R:GTTGACTGTACTTGGAAGAA
9,10,11 F: GAGGTGGGAACCAGTTTGATG 52
9,10,11 R: CAAGCCAATCTCTGTAGTGCG
12 F:GAGGCTTGATACATAGGC 52
12 R:CCATAAGACCAGGAGATT
13(1) F:GTTTCTGAGGGTGCTACT 48
13(1) R:TTGAATGGCTAACTGGG
13(2) F:CAGTTTCTGAAGGTGCTA 48
13(2) R:TCCATCCAGTCTACATTG
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