Table 1 of Yuan, Mol Vis 2007; 13:1555-1561.


Table 1. Oligonucleotides used for PAX6 amplification

Sequence of oligonucleotides and annealing temperatures used for exon-by-exon PAX6 amplification.In the table, "F" indicates the forward strand and "R" indicates the reverse strand. Exon 13 was amplified in two overlapping fragments. In each case, primer pair (1)F and(1)R amplifies the most upstream 5' part of the target genomic DNA, and primer pair, (2)F and (2)R, amplifies the most downstream 3' part of the target genomic DNA encompassing the specific exonic sequences. Sequences are given in the 5'-3' direction.

                                      Annealing
                                     temperature
 Exon         Sequences(5'-3')          (°C)
-------   ------------------------   -----------
3,4       F:GGACTTAGGGTTTGATGACAG        50
3,4       R:CCAGAAAGACCAGAGGCAC
5         F:GAGATTGGCACAGGTTGG           60
5         R:CATAAGTAGCATCGTTTACAGT
5a,6      F:AACGCCACTTTAAGCAAGGT         52
5a,6      R: GGAGGGCAGATGTTCTCAA
7         F:AATCCACCCACTGTCCCG           60
7         R:CCAGCCACCTTCATACCG
8         F:TCAGGTAACTAACATCGCA          53
8         R:GTTGACTGTACTTGGAAGAA
9,10,11   F: GAGGTGGGAACCAGTTTGATG       52
9,10,11   R: CAAGCCAATCTCTGTAGTGCG
12        F:GAGGCTTGATACATAGGC           52
12        R:CCATAAGACCAGGAGATT
13(1)     F:GTTTCTGAGGGTGCTACT           48
13(1)     R:TTGAATGGCTAACTGGG
13(2)     F:CAGTTTCTGAAGGTGCTA           48
13(2)     R:TCCATCCAGTCTACATTG

Yuan, Mol Vis 2007; 13:1555-1561 <http://www.molvis.org/molvis/v13/a172/>
©2007 Molecular Vision <http://www.molvis.org/molvis/>
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