Figure 3 of Martins, Mol Vis 2007; 13:142-150.

Figure 3. Agarose gel electrophoresis of glycosaminoglycans released by proteolysis from proteoglycans extracted from human corneal explants after LASIK

The proteoglycans extracted from pair number 12 (see Table 1) of human corneal explants underwent proteolysis with papain for release of the glycosaminoglycan chains. Aliquots of the incubation mixtures were submitted to agarose gel electrophoresis. Total proteoglycans (PG) and glycosaminoglycans (GAG) were stained by toluidine blue (A), and the 35S-labeled glycosaminoglycans (35S-GAG) were localized by radioautography (B). S: A mixture of standard glycosaminoglycans containing chondroitin sulfate (CS) dermatan sulfate (DS), and heparan sulfate (HS), 5 μg each; KS, keratan sulfate; PG, intact proteoglycans.

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Martins, Mol Vis 2007; 13:142-150 <>
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