Table 2 of
van Wijngaarden, Mol Vis 2007;
13:1508-1515.Table 2 of
van Wijngaarden, Mol Vis 2007;
13:1508-1515.
Table 2. Primer sequences of candidate reference genes for quantitative real-time reverse transcription polymerase chain reactions on rat retina
The following abbreviations were used: melting temperature (Tm), base pairs (bp), forward primer (F), and reverse primer (R).
Amplicon
Nucleotide Tm size
Gene (accession number) Primer sequence (5'-3') position (°C) (bp)
----------------------------------- ------------------------ ---------- -------- --------
Acidic ribosomal phosphoprotein F: AAAGGGTCCTGGCTTTGTCT 766-856 60 59 91
(ARBP) NM_022402.1 R: GCAAATGCAGATGGATCG
Actin gamma 2 (ACTG2) NM_012893.1 F: GGAGAAGATCTGGCACCACT 264-369 59 60 104
R: CCCTGTTGGCTTTAGGGTTT
Cyclophilin A (CYCA) NM_017101.1 F: GTCTGCTTCGAGCTGTTTGC 100-179 61 60 80
R: AATCCTTTCTCCCCAGTGCT
RNA polymerase 2 (RNAP2) F: GTCCAATGACATCGTGGAGA 4011-4106 59 60 96
XM_343922.2 R: CCATCAAAGGAGATGACATGG
Hypoxanthine guanine F: TTGTTGGATATGCCCTTGACT 629-733 59 60 105
phosphoribosyl-transferase (HPRT) R: CCGCTGTCTTTTAGGCTTTG
NM_012583.2
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