Table 1 of
van Wijngaarden, Mol Vis 2007;
13:1508-1515.Table 1 of
van Wijngaarden, Mol Vis 2007;
13:1508-1515.
Table 1. Reference genes used in other studies
The following abbreviations were used: acidic ribosomal phosphoprotein (ARBP), cyclophilin A (CYCA), glyceraldehyde-3-phosphate dehydrogenase, (GAPDH), hypoxanthine guanine phosphoribosyltransferase (HPRT), RNA polymerase II (RNAP2), succinate dehydrogenase complex, subunit A (SDHA).
Reference gene Study context Stability of gene expression Ref
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ARBP Hypercarbic OIR in rats ARBP expression stability not assessed. 30
Beta-actin; CYCA; GAPDH; 28S rRNA Direct comparison of reference gene expression 28S rRNA stably expressed in all cell lines 31
stability in 4 cultured cell lines in normoxia and irrespective of oxygen tension; GAPDH expression
hypoxia. induced strongly by hypoxia; variation in
expression of beta-actin and CYCA with hypoxia.
ARBP Normal murine post-natal retinal development. ARBP expression stable during development, minor 32
variation in expression between earliest
time-points (day 1 and day 3).
ARBP Mouse retina, brain, liver: RNA from mice of mixed ARBP expression stability not specifically 33
ages and genotypes. addressed.
ARBP; 28S rRNA; cyclophilin; GAPDH Murine OIR: measurement of gene expression after ARBP expression stable at all time-points; 28S 34
hyperoxia and during relative hypoxia. expression decreased slightly after 12 h of
relative hypoxia; expression stability of other
genes not stated.
Beta-actin; GAPDH; HPRT; SDHA Rat cultured cortical neurons: response to anoxia Expression stability of individual reference genes 35
with or without growth factors. not stated; geometric mean of all four genes used
for normalization.
Beta-actin; HPRT; GAPDH Adult rat retina made ischemic by brief episode HPRT and GAPDH showed stable expression; 36
of elevated intraocular pressure. beta-actin was upregulated in ischemia compared
with controls.
Beta-actin; CYCA; GAPDH Adult rat focal cerebral ischemia model. Significant down-regulation of CYCA and GAPDH with 37
ischemia; beta-actin expression increased.
CYCA Murine OIR: gene expression measured at 10 CYCA expression stable during normal retinal 38
time-points after birth. development; expression stability during and after
hyperoxia not stated.
GAPDH; 18S rRNA Neonatal rats exposed to hypoxia and No mention of reference gene expression stability 39
reoxygenation; brain, other tissues examined. but similar results attained after normalization
with either gene.
GAPDH Adult rat retina; ischemia/reperfusion injury. GAPDH expression stability not assessed. 40
Beta-actin; gamma-actin Development of rat brain from embryonic day 11 to Beta-actin significantly downregulated in neurons 41
adult; expression assessed at protein level. during normal development; gamma-actin
developmentally stable.
13 reference genes including RNAP2 Systematic comparison of 13 genes in 16 different RNAP2 stable expression in all tissues; little 5
human tissues under different conditions; change in expression in T cell line following
hyperoxia/hypoxia not examined. mitogenic stimulation.
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