Figure 2. Comparison of candidate gene amplicons on agarose gels after real-time reverse transcription polymerase chain reactions

Lane 1: ARBP (91 base-pair-bp); lane 2: RNAP2 (96 bp); lane 3: HPRT (105 bp); lane 4: ACTG2 (104 bp); lanes 5 and 6: markers-20 bp ladder; lane 7: CYCA. A single product of the expected size was amplified by all of the reference gene primer pairs tested, except for CYCA, where three products are marked by arrows. The predominant product was of the expected size (80 bp); the other two products were likely to have resulted from pseudogene amplification. The following abbreviations were used: cyclophilin A (CYCA), RNA polymerase 2 (RNAP2), acidic ribosomal phosphoprotein (ARBP), hypoxanthine guanine phosphoribosyltransferase (HPRT), and γ2 actin (ACTG2).