Figure 7 of
Cristofanilli, Mol Vis 2007;
13:1496-1507.Figure 7 of
Cristofanilli, Mol Vis 2007;
13:1496-1507.
Figure 7. Correlation between Cav1.3 channel internalization and inhibition of L-type Ca current by cytochalasin B in retinal ganglion cells
A: L-type Ca currents (ICa) recorded from ganglion cells with the patch pipette containing 10 μM cytB in control slices, and B in slices preincubated for 60 min with 50 μM dynamin inhibitory peptide (DIP). Traces are peak ICa recorded in response to a depolarizing step from -70 to 0 mV at indicated times after the membrane rupture. C: Corresponding normalized I-V relationships obtained from ganglion cells within 1-2 min of membrane rupture (closed circles), and after 20-25 min (open squares) in control slices, and D in slices pretreated with DIP. E: Time course of peak ICa changes by cytB in control and DIP-treated ganglion cells. F: The bar graph summarizes data, indicating that block of endocytosis by DIP attenuated the inhibitory action of cytB on L-type ICa. Columns represent the mean±SE (n=6, p<0.005).
