Figure 5. Scratch wound and transwell migration assays of CEC

Confluent monolayers of wild type or TSP1^-/- CEC (A) were wounded and wound closure was monitored after 72 h and photographed in digital format. Please note that the wild type CEC migrated and covered the wound, while significant area of the wound remains uncovered in TSP1^-/- CEC. B shows the migration of wild type and TSP1^-/- CEC through a transwell as described in Methods. A significant decrease in the mean number of TSP1^-/- CEC migrating through the filter compared to the wild type cells was observed (p<0.05). These experiments were performed at least twice with two different isolations of CEC.