Figure 4 of
Scheef, Mol Vis 2007;
13:1483-1495.
Figure 4. Western blot analysis of conditioned medium and cell lysates prepared from CEC
Levels of TSP1, TSP2, eNOS, nNOS, tenascin-C, and fibronectin were analyzed by western blot analysis of serum free-conditioned medium and cell lysates prepared from CEC as described in Methods. Please note the absence of TSP1 in TSP1-/- CEC. The TSP1-/- CEC express less fibronectin, both secreted and cell-associated, compared to wild type CEC. Tenascin-C was only detectable in TSP1-/- CEC. Both wild type and TSP1-/- CEC did not express detectable levels of TSP2 or eNOS, but expressed nNOS. The blot was also incubated with anti-β-catenin as a loading control for intercellular protein levels.