Figure 4. PRDX3 is induced in cultured lenses exposed to H₂O₂

PRDX3 transcript levels detected in rat lenses cultured in the presence (+) or absence of (-) of 50 μM H₂O₂ for 1 h and allowed to recover 12 h in serum-free, phenol-free media. (A) Representative H₂O₂-treated (+) and untreated control lenses (-). (B) Ethidium bromide stained agarose gel showing Rat PRDX3 transcript and corresponding rat ribosomal 28 S RNA control transcript detected by RT-PCR using 40 ng of total RNA and 40 RT-PCR cycles.