Figure 3. Oxidative stress induces PRDX3 mRNA and protein in HLE cells

A: Ethidium bromide stained agarose gel showing PRDX3 transcript levels in 100 ng RNA prepared from human lens epithelial cells exposed to 0 μM, 2 μM, 10 μM, and 50 μM H₂O₂ for 1 h. RNA was isolated after 12 h recovery period in the absence of H₂O₂ and RT-PCR was performed for 30 PCR cycles. B: Western blot showing PRDX3 protein levels detected using 1 μg of protein isolated from HLE cells exposed to 0 μM, 2 μM, 10 μM, 50 μM, and 100 μM for 1 h. Protein was isolated after a 36 h recovery period in the absence of H₂O₂. Densitometry values represent experimental (H₂O₂-treated) minus control (untreated) values.