Table 3 of
Bagiyeva, Mol Vis 2007;
13:1458-1468.
Table 3. Polymerase chain reaction primers and enzymes used for restriction fragment length polymorphisms cosegregation analyses in families bearing the mutations indicated
The restriction enzymes used for cosegregation analyses in families bearing the missense CYP1B1 mutations. The plus (+) or minus (-) symbol indicate gain or loss of the enzyme restriction site in the mutated sequence.
Mutations Restriction enzymes PCR primers Families --------- ------------------- ------------ ------------------------------- G61E TaqI (+) CYP2F//CYP2R PCG2; PCG6; PCG13; PCG20; PCG25 R469W AciI (-) CYP3F//3SeqR PCG9; PCG32; PCG35 R117W BseNI (+) CYP2F//2SeqR PCG9 G329V SfoI (-) CYP2F//CYP2R PCG21 |