Figure 3. Conservation of the R117 and G329 residues (numeration according to human CYP1B1), as shown by protein alignment of several CYP1B1 orthologues and CYP1A1 and CYP1A2 paralogues from different species

The most evolutionarily conserved residues are highlighted in black, while partially conserved residues are highlighted in light grey. The two mutated residues (arrows) are highly conserved. Protein sequences: DrCYP1A1- Danio rerio AAQ97766; OmCYP1A2- Oncorhynchus mykisss Q92110; MmCYP1A1- Mus musculus NP_034122; RnCYP1A1- Rattus norvegicus NP_036672; HsCYP1A1- Homo sapiens NP_000490; OcCYP1A1- Oryctolagus cuniculus P05176; CpCYP1A1- Cavia porcellus BAA01794; MmCYP1A2- Mus musculus NP_034123; RnCYP1A2- Rattus norvegicus NP_036673; CpCYP1A2- Cavia porcellus BAA09048; HsCYP1A2- Homo sapiens NP_000752; OcCYP1A2- Oryctolagus cuniculus P00187; GgCYP1A1- Gallus gallus NP_990477; MmCYP1B1- Mus musculus NP_034124; RnCYP1B1- Rattus norvegicus NP_037072; HsCYP1B1- Homo sapiens NP_000095; DrCYP1B1- Danio rerio NP_001013285.